Product nameAnti-Methionine Aminopeptidase 2/p67 antibody
See all Methionine Aminopeptidase 2/p67 primary antibodies
DescriptionRabbit polyclonal to Methionine Aminopeptidase 2/p67
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Human Methionine Aminopeptidase 2/p67 aa 100-150.
Database link: NP_006829.1
- HeLa, 293T, Jurkat, NIH3T3 whole cell lysates
This product was previously labelled as Methionine Aminopeptidase 2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab123523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 52 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
Sequence similaritiesBelongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
modificationsContains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.
Cellular localizationCytoplasm. About 30% of expressed METAP2 associates with polysomes.
- Information by UniProt
- A930035J23Rik antibody
- AI047573 antibody
- AL024412 antibody
Detection of Methionine Aminopeptidase 2/p67 by Western Blot of Immunprecipitate.
Lane 1: ab123523 at 1µg/ml staining Methionine Aminopeptidase 2/p67 in HeLa whole cell lysate immunoprecipitated using ab123523 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.
All lanes : Anti-Methionine Aminopeptidase 2/p67 antibody (ab123523) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 30 seconds
ab123523 has not yet been referenced specifically in any publications.