Recombinant Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887] (ab134124)


  • Product name

    Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887]
    See all Methionine Aminopeptidase 2/p67 primary antibodies
  • Description

    Rabbit monoclonal [EPR6887] to Methionine Aminopeptidase 2/p67
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Methionine Aminopeptidase 2/p67 aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa whole cell lysate (ab150035), Caco 2 cell lysate, HepG2 whole cell lysate (ab7900), Human colon tissue.
  • General notes



     This product was previously labelled as Methionine Aminopeptidase 2


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab134124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
      Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
    • Sequence similarities

      Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
    • Post-translational

      Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.
    • Cellular localization

      Cytoplasm. About 30% of expressed METAP2 associates with polysomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • A930035J23Rik antibody
      • AI047573 antibody
      • AL024412 antibody
      • Amp2 antibody
      • AU014659, antibody
      • EIF 2 associated p67 homolog antibody
      • EIF-2-associated p67 antibody
      • Initiation factor 2 associated 67 kDa glycoprotein antibody
      • Initiation factor 2-associated 67 kDa glycoprotein antibody
      • MAP 2 antibody
      • MAP2 antibody
      • MAP2_HUMAN antibody
      • MetAP 2 antibody
      • Metap2 antibody
      • Methionine aminopeptidase 2 antibody
      • Methionyl aminopeptidase 2 antibody
      • MGC102452 antibody
      • MGC127390 antibody
      • MGC53792 antibody
      • MNPEP antibody
      • p67 antibody
      • p67eIF2 antibody
      • Peptidase M antibody
      • Peptidase M2 antibody
      see all


    • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Methionine Aminopeptidase 2/p67 with ab134124 at 1/250.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Overlay histogram showing HeLa cells stained with ab134124 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134124, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887] (ab134124) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Caco 2 cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 67 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    ab134124 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134124.
    Please use the links above to contact us or submit feedback about this product.

    For licensing inquiries, please contact

    Sign up