Recombinant
RabMAb

Recombinant Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887] (ab134124)

Overview

  • Product name

    Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887]
    See all Methionine Aminopeptidase 2/p67 primary antibodies
  • Description

    Rabbit monoclonal [EPR6887] to Methionine Aminopeptidase 2/p67
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Methionine Aminopeptidase 2/p67 aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa whole cell lysate (ab150035), Caco 2 cell lysate, HepG2 whole cell lysate (ab7900), Human colon tissue.
  • General notes

     

     

     This product was previously labelled as Methionine Aminopeptidase 2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
      Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
    • Sequence similarities

      Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
    • Post-translational
      modifications

      Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.
    • Cellular localization

      Cytoplasm. About 30% of expressed METAP2 associates with polysomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • A930035J23Rik antibody
      • AI047573 antibody
      • AL024412 antibody
      • Amp2 antibody
      • AU014659, antibody
      • EIF 2 associated p67 homolog antibody
      • EIF-2-associated p67 antibody
      • Initiation factor 2 associated 67 kDa glycoprotein antibody
      • Initiation factor 2-associated 67 kDa glycoprotein antibody
      • MAP 2 antibody
      • MAP2 antibody
      • MAP2_HUMAN antibody
      • MetAP 2 antibody
      • Metap2 antibody
      • Methionine aminopeptidase 2 antibody
      • Methionyl aminopeptidase 2 antibody
      • MGC102452 antibody
      • MGC127390 antibody
      • MGC53792 antibody
      • MNPEP antibody
      • p67 antibody
      • p67eIF2 antibody
      • Peptidase M antibody
      • Peptidase M2 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Methionine Aminopeptidase 2/p67 with ab134124 at 1/250.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Overlay histogram showing HeLa cells stained with ab134124 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134124, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887] (ab134124) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Caco 2 cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 67 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab134124 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134124.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up