Overview

  • Product name

    Methionine Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Serum, Plasma
  • Assay type

    Quantitative
  • Product overview

    Methionine Assay Kit (Fluorometric) (ab234041) utilizes an enzymatic mechanism by which metabolism of methionine is coupled with the stoichiometric generation of hydrogen peroxide. A probe generates a fluorometric signal that can be quantified at Ex/Em: 535/587 nm. The assay shows greater than 10-fold specificity for methionine over cysteine and greater than 40-fold selectivity over other standard and non-standard amino acids. After utilization of a proprietary Sample Clean-Up Mix, the method is suitable for use in blood samples, and can detect concentrations as low as 0.5 µM, or 25 pmole Methionine.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Methionine Assay Buffer 1 x 25ml
    Methionine Buffer Supplement 1 vial
    Methionine Developer 1 x 200µl
    Methionine Enzyme Mix I 1 x 200µl
    Methionine Enzyme Mix II 1 vial
    Methionine Probe 1 x 200µl
    Methionine Standard (100 mM) 1 x 100µl
    Sample Clean-up Mix 1 vial
  • Research areas

  • Relevance

    Methionine is an essential amino acid, it cannot be synthesized in humans. However, in plants and microorganisms, methionine is synthesized from aspartic acid and cysteine. Methionine plays a role in cysteine, carnitine and taurine synthesis by the transsulfuration pathway, lecithin production, the synthesis of phosphatidylcholine and other phospholipids. Improper conversion of methionine can lead to atherosclerosis. Methionine is a chelating agent.
  • Alternative names

    • Met
    • Methionine

Images

  • The assay was run on various volumes of treated plasma and the results averaged. Methionine concentration was determined to be 18.6 µM in human plasma (2-12 µL; undiluted).

  • The assay was run in the presence of 1 nmole (1000 pmole) each of the amino acids Methionine, Cysteine, Homocysteine and Alanine. Numbers are relative to 100% response to methionine.

Protocols

References

ab234041 has not yet been referenced specifically in any publications.

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