Overview

  • Product name
    Anti-Methionine Sulfoxide Reductase A antibody
    See all Methionine Sulfoxide Reductase A primary antibodies
  • Description
    Rabbit polyclonal to Methionine Sulfoxide Reductase A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Macaque monkey
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • Mouse kidney extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab16803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 26 kDa.
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function
    Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.
  • Tissue specificity
    Ubiquitous. Highest expression in adult kidney and cerebellum, followed by liver, heart ventricles, bone marrow and hippocampus.
  • Sequence similarities
    Belongs to the MsrA Met sulfoxide reductase family.
  • Cellular localization
    Cytoplasm; Cytoplasm. Nucleus and Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytosolic methionine S sulfoxide reductase antibody
    • Methionine sulphoxide reductase A antibody
    • Mitochondrial peptide methionine sulfoxide reductase antibody
    • MSR A antibody
    • msrA antibody
    • MSRA_HUMAN antibody
    • peptide met (O) reductase antibody
    • Peptide Met(O) reductase antibody
    • Peptide methionine (S) S oxide reductase antibody
    • Peptide-methionine (S)-S-oxide reductase antibody
    • PMSR antibody
    • Protein methionine S oxide reductase antibody
    • Protein-methionine-S-oxide reductase antibody
    see all

Images

  • All lanes : Anti-Methionine Sulfoxide Reductase A antibody (ab16803)

    Lane 1 : Mouse Kidney
    Lane 2 : Rat Kidney

    Predicted band size: 26 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?



    Western blot using ab16803 at 1/2000.

    Lane 1: Mouse kidney
    Lane 2: Rat kidney

  • ab16803 (1µg/ml) staining Methionine Sulfoxide Reductase A in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartment of the intestinal glands cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:
  • Steen KA  et al. FABP4/aP2 Regulates Macrophage Redox Signaling and Inflammasome Activation via Control of UCP2. Mol Cell Biol 37:N/A (2017). WB ; Mouse . Read more (PubMed: 27795298) »
  • Liu Y  et al. Astragaloside IV rescues MPP+-induced mitochondrial dysfunction through upregulation of methionine sulfoxide reductase A. Exp Ther Med 14:2650-2656 (2017). Read more (PubMed: 28962208) »
See all 9 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question

We have purchased your primary antibodies: ab16803 and ab71175. We read the product information in your official website, and find some suspicious places. So we need your detailed interpretation.



1. In one “Specific reference” of ab16803, TXNL6 Is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of a-Crystallin and Cytochrome C in the Eye Lens, there is one sentence in “SDS-PAGE and western blotting”: “primary anti-MsrA (Abcam) 1:2000”. But the author didn’t tell us the exact product code of it in the full text. Please inform us the exact product code and catalog number of this primary anti-MsrA antibody the author used.



2. In another “Specific reference” of ab16803, Neutrophil granulocytes uniquely express, among human blood cells, high levels of Methionine-sulfoxide-reductase enzymes, there is one sentence in “Antibodies and chemicals”: “rabbit polyclonal anti-MsrB1 (ab16804)”. But we can’t find “ab16804” in your official website. What is the reason for it?



3. In the product introduction of ab71175, https://www.abcam.com/Methionine-Sulfoxide-Reductase-B-antibody-ab71175.html, one of “Anti-Methionine Sulfoxide Reductase B antibody images”, there is a watermark “gln” in the blue image. So why is there “gln” in the blue image? Who, which research institute or which company finished this Western blot and then you quoted it? When was this Western blot image finished? Can you give us the detailed origin and paper information (title, author, Journal name, etc.) of this image?

Read More
Answer

Thank you for contacting us.

The product code of the antibody that was used in the reference listed on the ab16803 datasheet was, as far as we know, ab16803. I believe this information,which is not in the text of the paper, was given to us by one of the authors, but I will confirm.


Antibody ab16804 is no longer in our catalogue, and is no longer produced.


The watermark on the western blot image on the ab71175 datasheet is from our source of this antibody, who produced the western blot.The image has not, to our knowledge, been published in any journal. I am not able to reveal the identity of the laboratory, or the sources of any of our antibodies.


If you have any other questions, please let me know.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Cow Recombinant protein (None)
Loading amount
0.15 µg
Specification
None
Gel Running Conditions
Reduced Denaturing (4-20% Gradient Pre-cast Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

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Verified customer

Submitted Jul 29 2010

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