Product nameAnti-Methionine Sulfoxide Reductase A antibody
See all Methionine Sulfoxide Reductase A primary antibodies
DescriptionRabbit polyclonal to Methionine Sulfoxide Reductase A
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Predicted to work with: Macaque monkey
Recombinant full length protein (Human).
- Mouse kidney extract.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab16803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 26 kDa.|
|IHC-P||Use a concentration of 1 µg/ml.|
FunctionHas an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.
Tissue specificityUbiquitous. Highest expression in adult kidney and cerebellum, followed by liver, heart ventricles, bone marrow and hippocampus.
Sequence similaritiesBelongs to the MsrA Met sulfoxide reductase family.
Cellular localizationCytoplasm; Cytoplasm. Nucleus and Mitochondrion.
- Information by UniProt
- Cytosolic methionine S sulfoxide reductase antibody
- Methionine sulphoxide reductase A antibody
- Mitochondrial peptide methionine sulfoxide reductase antibody
All lanes : Anti-Methionine Sulfoxide Reductase A antibody (ab16803)
Lane 1 : Mouse Kidney
Lane 2 : Rat Kidney
Predicted band size: 26 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Western blot using ab16803 at 1/2000.
Lane 1: Mouse kidney
Lane 2: Rat kidney
ab16803 (1µg/ml) staining Methionine Sulfoxide Reductase A in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartment of the intestinal glands cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
This product has been referenced in:
- Steen KA et al. FABP4/aP2 Regulates Macrophage Redox Signaling and Inflammasome Activation via Control of UCP2. Mol Cell Biol 37:N/A (2017). WB ; Mouse . Read more (PubMed: 27795298) »
- Liu Y et al. Astragaloside IV rescues MPP+-induced mitochondrial dysfunction through upregulation of methionine sulfoxide reductase A. Exp Ther Med 14:2650-2656 (2017). Read more (PubMed: 28962208) »