Recombinant
RabMAb

Recombinant Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

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Overview

  • Product name

    Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)]
    See all Methionyl Aminopeptidase 1/MAP 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15452(B)] to Methionyl Aminopeptidase 1/MAP 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Methionyl Aminopeptidase 1/MAP 1 aa 100-200. The exact sequence is proprietary.
    Database link: P53582

  • Positive control

    • A375 and HUVEC lysate. Human cervix carcinoma and Rat skeletal muscle tissue. A549 and A375 cells.

  • General notes

     

     

     This product was previously labelled as Methionyl Aminopeptidase 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250.
Flow Cyt 1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Flow Cytometry - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Flow Cytometry - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Flow cytometry analysis of 2% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/180 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Immunofluorescent analysis of 4% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Counterstained with Dapi blue.

  • Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    All lanes : Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540) at 1/1000 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

References

ab185540 has not yet been referenced specifically in any publications.

Publishing research using ab185540? Please let us know so that we can cite the reference in this datasheet.

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