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RecombinantRabMAb

Recombinant Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
  • Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
  • Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
  • Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
  • Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15452(B)] to Methionyl Aminopeptidase 1/MAP 1
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Protein
Product image
Recombinant Human Methionyl Aminopeptidase 1/MAP 1 protein (ab124578)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)]
    See all Methionyl Aminopeptidase 1/MAP 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15452(B)] to Methionyl Aminopeptidase 1/MAP 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Methionyl Aminopeptidase 1/MAP 1 aa 100-200. The exact sequence is proprietary.
    Database link: P53582

  • Positive control

    • A375 and HUVEC lysate. Human cervix carcinoma and Rat skeletal muscle tissue. A549 and A375 cells.
  • General notes

     

     

     This product was previously labelled as Methionyl Aminopeptidase 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15452(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (ab250831)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Methionyl Aminopeptidase 1/MAP 1 protein (ab124578)
  • Related Products

    • Recombinant Human Methionyl Aminopeptidase 1/MAP 1 protein (ab124578)

Applications

Our Abpromise guarantee covers the use of ab185540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250.
Flow Cyt 1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Removes the amino-terminal methionine from nascent proteins. Required for normal progression through the cell cycle.
  • Sequence similarities

    Belongs to the peptidase M24A family.
  • Target information above from: UniProt accession P53582 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23173 Human
    • Entrez Gene: 75624 Mouse
    • Entrez Gene: 295500 Rat
    • Omim: 610151 Human
    • SwissProt: P53582 Human
    • SwissProt: Q8BP48 Mouse
    • Unigene: 480364 Human
    • Unigene: 26833 Mouse
    • Unigene: 6799 Rat
    see all
  • Alternative names

    • AMPM1_HUMAN antibody
    • DKFZp781C0419 antibody
    • KIAA0094 antibody
    • MAP 1 antibody
    • MAP1A antibody
    • MetAP 1 antibody
    • metap1 antibody
    • MetAP1A antibody
    • Methionine aminopeptidase 1 antibody
    • Peptidase M 1 antibody
    see all

Images

  • Flow Cytometry - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Flow Cytometry - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Flow cytometry analysis of 2% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/180 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Immunofluorescent analysis of 4% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Counterstained with Dapi blue.

  • Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    All lanes : Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540) at 1/1000 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

  • Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab185540? Please let us know so that we can cite the reference in this datasheet.

    ab185540 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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