Product nameAnti-Methionyl tRNA synthetase antibody
See all Methionyl tRNA synthetase primary antibodies
DescriptionRabbit polyclonal to Methionyl tRNA synthetase
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey, Rhesus monkey
- HeLa, Jurkat, and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 101 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 GST C-terminal domain.
Contains 1 WHEP-TRS domain.
- Information by UniProt
- cytoplasmic antibody
- FLJ35667 antibody
- Mars antibody
All lanes : Anti-Methionyl tRNA synthetase antibody (ab157115) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 101 kDa
Exposure time: 30 seconds
Immunoprecipitation analysis of whole cell lysate from 293T cells (1 mg for IP; 20% of IP loaded), labeling Methionyl tRNA synthetase with ab157115 at 6 µg/mg lysate (lane 1). Immunoprecipitation of Methionyl tRNA synthetase was also tested using an alternative Rabbit Methionyl tRNA synthetase (lane 2), or control IgG (lane 3). For blotting immunoprecipitated Methionyl tRNA synthetase, ab157115 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
ab157115 has not yet been referenced specifically in any publications.