Anti-Methionyl tRNA synthetase antibody (ab157115)

Overview

  • Product name
    Anti-Methionyl tRNA synthetase antibody
    See all Methionyl tRNA synthetase primary antibodies
  • Description
    Rabbit polyclonal to Methionyl tRNA synthetase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 175-225 of Human Methionyl tRNA synthetase according to (NP_004981.2).

  • Positive control
    • HeLa, Jurkat, and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab157115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 101 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Sequence similarities
    Belongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 GST C-terminal domain.
    Contains 1 WHEP-TRS domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic antibody
    • FLJ35667 antibody
    • Mars antibody
    • Methionine tRNA ligase 1 antibody
    • Methionine tRNA ligase 1 cytoplasmic antibody
    • Methionine tRNA ligase antibody
    • Methionine tRNA synthetase antibody
    • Methionine--tRNA ligase antibody
    • Methionyl tRNA synthetase cytoplasmic antibody
    • Methionyl-tRNA synthetase antibody
    • MetRS antibody
    • MRS antibody
    • MTRNS antibody
    • SYMC_HUMAN antibody
    see all

Images

  • All lanes : Anti-Methionyl tRNA synthetase antibody (ab157115) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 101 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation analysis of whole cell lysate from 293T cells (1 mg for IP; 20% of IP loaded), labeling Methionyl tRNA synthetase with ab157115 at 6 µg/mg lysate (lane 1). Immunoprecipitation of Methionyl tRNA synthetase was also tested using an alternative Rabbit Methionyl tRNA synthetase (lane 2), or control IgG (lane 3). For blotting immunoprecipitated Methionyl tRNA synthetase, ab157115 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

References

ab157115 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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