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Rabbit polyclonal to Methionyl tRNA synthetase
Predicted to work with:
Cynomolgus monkey, Rhesus monkey
Synthetic peptide corresponding to a region within amino acids 175-225 of Human Methionyl tRNA synthetase according to (NP_004981.2).
HeLa, Jurkat, and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 101 kDa.
Use at 2-10 µg/mg of lysate.
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 GST C-terminal domain. Contains 1 WHEP-TRS domain.
Information by UniProt
Western blot - Anti-Methionyl tRNA synthetase antibody (ab157115)
All lanes : Anti-Methionyl tRNA synthetase antibody (ab157115) at 0.1 µg/ml Lane 1 : 293T whole cell lysate Lane 2 : HeLa whole cell lysate Lane 3 : Jurkat whole cell lysate Lysates/proteins at 50 µg per lane. Developed using the ECL technique. Predicted band size: 101 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-Methionyl tRNA synthetase antibody (ab157115)
Immunoprecipitation analysis of whole cell lysate from 293T cells (1 mg for IP; 20% of IP loaded), labeling Methionyl tRNA synthetase with ab157115 at 6 µg/mg lysate (lane 1). Immunoprecipitation of Methionyl tRNA synthetase was also tested using an alternative Rabbit Methionyl tRNA synthetase (lane 2), or control IgG (lane 3). For blotting immunoprecipitated Methionyl tRNA synthetase, ab157115
was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
has not yet been referenced specifically in any publications.
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