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Rabbit polyclonal to Methionyl tRNA synthetase
Predicted to work with:
Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset
Synthetic peptide, corresponding to a region within amino acids 650-700 of Human Methionyl tRNA synthetase (NP_004981.2).
293T, HeLa, Jurkat, mouse TCMK 1 and mouse NIH3T3 cells whole cell lysates.
Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate Note: pH 7-8.
Concentration information loading...
Immunogen affinity purified
ab157119 was affinity purified using an epitope specific to Methionyl tRNA synthetase immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 101 kDa.
Use at 2-10 µg/mg of lysate.
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 GST C-terminal domain. Contains 1 WHEP-TRS domain.
Information by UniProt
Western blot - Anti-Methionyl tRNA synthetase antibody (ab157119)
All lanes : Anti-Methionyl tRNA synthetase antibody (ab157119) at 0.4 µg/ml Lane 1 : 293T whole cell lysate Lane 2 : HeLa whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : Mouse TCMK1 whole cell lysate Lane 5 : Mouse NIH3T3 whole cell lysate Lysates/proteins at 50 µg per lane. Developed using the ECL technique. Predicted band size: 101 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-Methionyl tRNA synthetase antibody (ab157119)
Detection of Methionyl tRNA synthetase by Western Blot of Immunprecipitate.
ab157119 at 1µg/ml labeling Methionyl tRNA synthetase in 293T whole cell lysate immunoprecipitated using ab157119 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of
has not yet been referenced specifically in any publications.
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