• Product name
    Anti-Methotrexate antibody
  • Description
    Goat polyclonal to Methotrexate
  • Host species
  • Specificity
    The antibody is highly specific for Methotrexate, but cross-reactivities have not been tested.
  • Tested applications
    Suitable for: ELISA, RIAmore details
  • Immunogen

    Pure Methotrexate conjugated to hemocyanin



Our Abpromise guarantee covers the use of ab2328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.


  • Relevance
    Methotrexate (rINN) abbreviated MTX and formerly known as amethopterin, is an antimetabolite and antifolate drug used in treatment of cancer, autoimmune diseases and as an abortifacient in the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Methotrexate competitively inhibits dihydrofolate reductase (DHFR), an enzyme that participates in the tetrahydrofolate synthesis. The affinity of methotrexate for DHFR is about one thousand-fold that of folate for DHFR. Methotrexate acts specifically during DNA and RNA synthesis, and thus it is cytotoxic during the S-phase of the cell cycle. Logically, it therefore has a greater toxic effect on rapidly dividing cells which replicate their DNA more frequently, and thus inhibits the growth and proliferation of these non-cancerous cells. Lower doses of methotrexate have been shown to be very effective for the management of rheumatoid arthritis, Crohn's disease, and psoriasis.
  • Alternative names
    • Amethopterin antibody
    • MTX antibody


ab2328 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for your email and kind answer. Indeed, I have found the below publication where the IgG content of normal adult goat serum was about 20mg/ml. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1445503/ Also, yes, only the specific antibodies should bind to the methotrexate. However there can be unspecific binding of other proteins if the conditions are not optimized (not enough washings for example). I hope this information is helpful. Please do not hesitate to contact us if you have any other question.

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Thank you for your enquiry. I apologise for the delay in responding to your message. Since it is the antibody directed against methotrexate that you need to purify, you will not need to include the hemocyanin. IgG from the sample can be purified non-specifically using protein A or G, protocols for this will be supplied from manufacturers. If you use immunogen-specific purification, as you have requested information on, this will result in IgG specific for that antigen. There is no specific protocol available for methotrexate, but several are available in the literature I have attached a link to one which I hope you will find useful. http://www.embl.de/ExternalInfo/pepperko/assets/applets/PDF-AntibodyPurification.pdf I hope this information is helpful. Please do not hesitate to contact us again should you have any more questions.

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Thank you for your enquiry. This antibody is not part of a kit, but we do have suggestions for compatible secondary antibodies and other products that may be helpful. These are available on the "Related Products" tab on the datasheet. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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