Key features and details
- Assay time: 4 hr
- Sample type: Adherent cells, Suspension cells
Product nameMethylated DNA Immunoprecipitation (MeDIP) ChIP Kit
See all 5-methylcytosine kits
Sample typeAdherent cells, Suspension cells
Assay time4h 00m
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.
Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135) enables the user to enrich methylated DNA directly from cells by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.
Storage instructionsPlease refer to protocols.
Components 24 tests 48 tests 8-Well Assay Strips (with Frame) 3 units 6 units 8-Well Strip Caps 3 units 6 units Anti-5-Methylcytosine (1 mg/mL) 1 x 25µl 1 x 50µl Antibody Buffer 1 x 15ml 1 x 30ml Binding Buffer 1 x 5ml 1 x 8ml ChIP Dilution Buffer 1 x 4ml 1 x 6ml DNA Release Buffer 1 x 2ml 2 x 2ml Elution Buffer 1 x 0.6ml 1 x 1.2ml F-Collection Tube 30 units 50 units F-Spin Column 30 units 50 units Lysis Buffer 1 x 4ml 1 x 6ml Normal Mouse IgG (1 mg/mL) 1 x 10µl 1 x 15µl Pre-Lysis Buffer 1 x 4ml 1 x 8ml Proteinase K (10 mg/mL) 1 x 25µl 1 x 50µl Reverse Buffer 1 x 2ml 2 x 2ml Wash Buffer 1 x 28ml 2 x 28ml
- 5 mC
- 5 MethylCytosine
ab117135 has been referenced in 2 publications.
- Ni WJ & Leng XM Down-regulated miR-495 can target programmed cell death 10 in ankylosing spondylitis. Mol Med 26:50 (2020). PubMed: 32450789
- Alahari S et al. The von Hippel Lindau tumour suppressor gene is a novel target of E2F4-mediated transcriptional repression in preeclampsia. Biochim Biophys Acta Mol Basis Dis N/A:N/A (2018). PubMed: 30053437