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Thank you very much for your reply. However, I still have a couple of questions regarding the Episeeker kits: ab 117136, 117144, 117145, 117147, 117150, 117151. 1.- Is the ChIPed DNA suitable for sequencing (for the typical ChIPSeq experiments)? 2.- On the last point: I do not clearly see the amount that you IP, since in all the kits before performing the ChIP you say 'dilute the required volume of supernatant with EMH4 or ECA4' but, how do you know the exact 'required volume'?? This I do not understand.
Asked on Jan 11 2012
1. Yes, the ChIPed DNA is suitable for ChIP-Seq. Some customers report having improved ChIP-Seq results when the ChIPed DNA is further purified via phenol/chloroform extraction and ethanol precipitation. 2. The 'required volume' is unfortunately vague by necessity, as the volume required must be determined empirically. I hope this is helpful. Please contact me again if you have any further questions.
Answered on Jan 11 2012