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Dear technical staff, I am very interested in some of your Episeeker kits, in the ones that are focused in animal tissues, since I mostly work only with mice. Regarding these kits, I am wondering what are your thoughts on using them on adipose tissue (either brown and/or white adipose tissue), since I am working mostly on this tissue. You claim that they should be ok in a variety of mammalian tissues, but I found that adipose tissue is a quite different tissue from the rest. Therefore, if you have experience on adipose samples, are there some hints in the protocol that I should be aware of? Amount to be used? Your comments are very important to me. - Moreover, in general, what is the typical yield that you obtain after a standard ChIP using these kits, in any tissue? Is the DNA obtained of enough quantity and quality to perform sequencing? - I do not clearly see the amount that you IP, since in all the kits before performing the ChIP you say 'dilute the required volume of supernatant with EMH4 or ECA4' but, how do you know the exact 'required volume'?? This I do not understand. Thank you very much, Merry Christmas!
Asked on Jan 11 2012