Overview

  • Product name
    Methylated DNA Quantification Kit (Colorimetric)
    See all 5-methylcytosine kits
  • Detection method
    Colorimetric
  • Sample type
    Saliva, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    >= 0.2 ng/well
  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Species independent
  • Product overview

    DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.

    Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

    It can be paired to use with Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130), which specifically quantifies 5-hmC, for simultaneous quantification of both methylated and hydroxymethylated DNA.

  • Platform
    Microplate reader

Properties

Images

  • Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117128. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117128.

Protocols

References

This product has been referenced in:
  • Amin M  et al. Cardiotrophic Growth Factor-Driven Induction of Human Muse Cells Into Cardiomyocyte-Like Phenotype. Cell Transplant 27:285-298 (2018). Read more (PubMed: 29637816) »
  • Li T  et al. Structural and mechanistic insights into UHRF1-mediated DNMT1 activation in the maintenance DNA methylation. Nucleic Acids Res 46:3218-3231 (2018). Functional Studies ; Mouse . Read more (PubMed: 29471350) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your reply.

Tween at 0.05 - 0.1% should be used.

I hope this information helps.

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Answer


I am very pleased to hear you would like to accept our offer and test ab117128 with "Flatworm species". This code will give you €350 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Flatworm and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

Please note that we do not have any Abreview page online for ab117128 which means you cannot submit an Abreview. Please contact me with protocol details, samples information and results details, I will then be adding the Abreview manually on the datasheet. Once you submit the Abreview the code will be activated for you to get a product worth of €350.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Answer

Thank you for your inquiry.

I heard back from the lab that generally, PBS-T buffer may be used for washing, however for the best results, they recommend to use the wash buffer included in the kit. We could get the washing buffer (either 14 mL or 28 mL) added the catalogue for you to purchase, but this could take up to a week. If you would like for me to add it, just let me know which size you'd need and then I can tell you what the price and availability would be.

I hope this information helps. Please contact us with any other questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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