Methylated DNA Quantification Kit (Fluorometric) (ab117129)


  • Product name
    Methylated DNA Quantification Kit (Fluorometric)
    See all 5-methylcytosine kits
  • Sample type
    Saliva, Urine, Plasma, Tissue, Adherent cells, Suspension cells
  • Assay type
  • Sensitivity
    >= 50 pg/well
  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Species independent
  • Product overview

    DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.

    Abcam's Methylated DNA Quantification Kit (Fluorometric) (ab117129) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

    It can be paired to use with Hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131), which specifically quantifies 5-hmC, for simultaneously quantification of both methylated and hydroxymethylated DNA.


  • Storage instructions
    Please refer to protocols.
  • Components 48 tests 96 tests
    10X Wash Buffer 1 x 14ml 1 x 28ml
    8-Well Assay Strips (with Frame) 6 units 12 units
    Binding Solution 1 x 5ml 1 x 10ml
    Capture Antibody, 1000 µg/mL 1 x 4µl 1 x 8µl
    Detection Antibody, 400 µg/mL 1 x 8µl 1 x 16µl
    Enhancer Solution 1 x 8µl 1 x 16µl
    Fluoro Developer 1 x 8µl 1 x 16µl
    Fluoro Dilutor 1 x 4ml 1 x 8ml
    Fluoro Enhancer 1 x 8µl 1 x 16µl
    Negative Control, 20 µg/mL 1 x 10µl 1 x 20µl
    Positive Control, 20 µg/mL 1 x 10µl 1 x 20µl
  • Research areas


  • Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117129. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117129.



This product has been referenced in:
  • Pinzón-Cortés JA  et al. Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation. Endocr Connect 6:708-725 (2017). Read more (PubMed: 28993426) »

See 1 Publication for this product

Customer reviews and Q&As

Having reviewed this case with the lab, I would like to offer some suggestions to help optimize the results from ab117129.

It appears that the signal may be saturated either due to

1) insufficient washing or
2) too much ti...

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Thank you for your enquiry and interest regarding ab117129.
I can confirm that the TE buffer specified in the protocol is constituted with 10 mM Tris-HCl pH 7.5-8 and 1 mM EDTA.
If you need any further assistance in the future, please do not ...

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Thank you for contacting Abcam.

Yes, the assay detect 5-mC on both single and double stranded DNA with the nearly same sensitivity. The standard is double stranded DNA.

Please let me know if there is anything else I can...

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Thank you for contacting us.

Your credit note ID is ******.

I am sorry that this kit did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of...

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Thank you very much for your patience.

We have now received the stimulation graph from the laboratory. Please find it in the attachment. Based on that graph, I am happy to confirm that stimulation at 550nm is absolutely fine, as well as emiss...

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I would like to inform you that we are still investigating the problem you experienced with the kits. The lab has already provided the following information: "The reading can be carried out at 530/590 nm +/- 10 generally. Thus, emission at 59...

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