Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 4 hr
- Sample type: Adherent cells, Plasma, Saliva, Suspension cells, Tissue, Urine
- Sensitivity: 50 pg/well
Product nameMethylated DNA Quantification Kit (Fluorometric)
See all 5-methylcytosine kits
Sample typeSaliva, Urine, Plasma, Tissue, Adherent cells, Suspension cells
Sensitivity>= 50 pg/well
Assay time4h 00m
Species reactivityReacts with: Species independent
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.
Abcam's Methylated DNA Quantification Kit (Fluorometric) (ab117129) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.
It can be paired to use with Hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131), which specifically quantifies 5-hmC, for simultaneously quantification of both methylated and hydroxymethylated DNA.
Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Binding Solution 1 x 5ml 1 x 10ml Capture Antibody, 1000 µg/mL 1 x 4µl 1 x 8µl Detection Antibody, 400 µg/mL 1 x 8µl 1 x 16µl Enhancer Solution 1 x 8µl 1 x 16µl Fluoro Developer 1 x 8µl 1 x 16µl Fluoro Dilutor 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 8µl 1 x 16µl Negative Control, 20 µg/mL 1 x 10µl 1 x 20µl Positive Control, 20 µg/mL 1 x 10µl 1 x 20µl
- 5 mC
- 5 MethylCytosine
Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117129. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117129.
ab117129 has been referenced in 1 publication.
- Pinzón-Cortés JA et al. Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation. Endocr Connect 6:708-725 (2017). PubMed: 28993426