Overview

  • Product name

    Anti-Methylated Lysine antibody
    See all Methylated Lysine primary antibodies
  • Description

    Rabbit polyclonal to Methylated Lysine
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Methylated KLH conjugates

  • Positive control

    • WB: protein trimethylated from human melanoma cells. IHC-P: Human breast tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab76118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.
WB 1/1000.

Target

  • Relevance

    Many proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains.
  • Alternative names

    • Dimethylysine antibody
    • Methyl lysine antibody
    • N epsilon dimethyl lysine antibody
    • N epsilon mono methyllysine antibody
    • N epsilon trimethyl lysine antibody
    • N(epsilon) trimethyl lysine antibody
    • Trimethyl lysine antibody
    see all

Images

  • Western blot analysis of protein trimethylated from human melanoma cell (MMRU) labeling Methylated Lysine with ab76118.
    Left: without competitive inhibition with N-(epsilon) Tri-methyl lysine.
    Right: competitive inhibition with N-(epsilon) Tri-methyl lysine (50 μg/ml).

  • ab76118 staining Methylated Lysine in Human Breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

This product has been referenced in:

  • Shimazu T  et al. Role of METTL20 in regulating ß-oxidation and heat production in mice under fasting or ketogenic conditions. Sci Rep 8:1179 (2018). Read more (PubMed: 29352221) »
  • Yao R  et al. Lysines 207 and 325 methylation of WDR5 catalyzed by SETD6 promotes breast cancer cell proliferation and migration. Oncol Rep 40:3069-3077 (2018). Read more (PubMed: 30226578) »
See all 9 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.
I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial with the order number of xxxxxx.
Could you please make sure that the customer centrifuges the vial containing liquid upon receipt in order to remove all the liquid which may be lodged in the cap.
We recommend centrifuging for 10,000 x g for 20 seconds to pull down the solution that might be trapped in the threads of the vial and under the lid. For smaller vials please place them within a microcentrifuge tube first before centrifuging.
Please do not hesitate to contact us if you need anything further.

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Answer

Thank you for your message. You mentions ab80182 as well as ab76118 at the beginning of this email. I assume you are meant to refer to datasheet of ab76118 only, not ab80182? I apologise for any confusion. I have been in contact wtih our source in order to clarify this information. They have confirmed we were regrettably originally sent incorrect information for the previous enquiry. I can confirm that this antibody will not cross react with acetylated proteins, mono-methylated proteins, or di-methylated proteins. We aim to provide accurate and correct information to customers, and I am sincerely sorry there was some confusion in this case. I have removed the published enquiry that had the incorrect details on the datasheet. With regards to ab7315, I am sorry this did not come up in my previous search on our system for you. I can confirm that this is tested in IP, and wil be suitable for this application. Thank you for your understanding. If you have any further questions, please do not hesitate to contact us. 

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