Overview

  • Product name

    Anti-Methylated Lysine antibody (Biotin)
    See all Methylated Lysine primary antibodies
  • Description

    Rabbit polyclonal to Methylated Lysine (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Specificity

    ab80182 detects proteins containing methylated lysine residues.
  • Tested applications

    Suitable for: WB, IP, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Methylated KLH conjugates

  • Positive control

    • Melanoma cells in TBSt.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab80182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    Many proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains.
  • Alternative names

    • Dimethylysine antibody
    • Methyl lysine antibody
    • N epsilon dimethyl lysine antibody
    • N epsilon mono methyllysine antibody
    • N epsilon trimethyl lysine antibody
    • N(epsilon) trimethyl lysine antibody
    • Trimethyl lysine antibody
    see all

References

ab80182 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply. I can confirm that protein A purification should work ok with ab80182 Anti-Methylated Lysine antibody.     We do have one other methylated lysine antibody that is tested and guaranteed for IP: ab76118 Rabbit polyclonal Anti-Methylated Lysine antibody Tested in: IP, WB, ELISA, ICC/IF, IHC-Fr Click here (or use the following: https://www.abcam.com/index.html?datasheet=76118). I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact me.       

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Answer

Thank you for your enquiry. I can confirm that ab80182 Methylated Lysine antibody  has been tested and recognizes mono and di methylated states. I am sorry Tri methylation has not yet been specifically tested. I can also confirm that this antibody has been tested in IP, as stated on the datasheet. All tested applications  and species are listed on the product datasheets, and these applications and species are covered by the Abpromise guarantee for 6 months after purchase. I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.  

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