Key features and details
- Rabbit polyclonal to Methylated Lysine (FITC)
- Suitable for: ICC/IF
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Product nameAnti-Methylated Lysine antibody (FITC)
See all Methylated Lysine primary antibodies
DescriptionRabbit polyclonal to Methylated Lysine (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificityThis antibody recognizes proteins methylated on lysine residues (mono and di-methylysine). It does not cross-react with acetylated proteins. Cross-reactivity to trimethyllysine has not been tested.
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Species independent
Methylated protein [s] conjugated to KLH.
- Human melanoma cells (MMRU).
FITC/Ab molar ratio: approximately 10:1
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was affinity purified using N epsilon-monomethyllysine and dimethyllysine on Agarose as an affinity matrix.
Our Abpromise guarantee covers the use of ab53527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
RelevanceMany proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains.
- Dimethylysine antibody
- Methyl lysine antibody
- N epsilon dimethyl lysine antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab53527 has not yet been referenced specifically in any publications.