Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)

Abreviews (8)Q&A (16)Specific References (19)

Overview

  • Product name
    Anti-Methylated Lysine (di methyl , mono methyl ) antibody
    See all Methylated Lysine primary antibodies
  • Description
    Rabbit polyclonal to Methylated Lysine (di methyl , mono methyl )
  • Host species
    Rabbit
  • Specificity
    Recognize proteins methylated on lysine residues (mono- & di-methyl lysine). This antibody was affinity purified with mono-methyl lysine and di-methyl lysine, and may also react with tri-methyl lysine. Does not cross-react with acetylated protein.
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Methylated Lysine conjugated to KLH.

  • Positive control
    • HCL (0.1N) extracted and acetone precipited histone fraction

Properties

Applications

Our Abpromise guarantee covers the use of ab23366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Do not use milk for any of the buffers as complex milk proteins may contain competitive methylated proteins. Use BSA instead. ab23366 incubation time 2h RT. If no or weak signal is obtained overnight at 4deg might help.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Many proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains.
  • Alternative names
    • Dimethylysine antibody
    • Methyl lysine antibody
    • N epsilon dimethyl lysine antibody
    • N epsilon mono methyllysine antibody
    • N epsilon trimethyl lysine antibody
    • N(epsilon) trimethyl lysine antibody
    • Trimethyl lysine antibody
    see all

Images

  • Western blot - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)
    Western blot - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)
    Lane 1 : Anti-Methylated Lysine (tri methyl) antibody (does not cross react with mono, or di methyl lysine residues)
    Lane 2 : Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)

    All lanes : Yeast extract
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)This image is courtesy of an anonymous Abreview
    ab23366 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed a heat mediated antigen retrieval step was performed in citrate buffer. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)This image is courtesy of an anonymous Abreview

    ab23366 staining formalin fixed paraffin-embedded rat kidney tissue sections.  The section was subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 5% serum for 30 minutes at 20°C, prior to incubation with the primary antibody (diluted 1/200 in PBS + 5% serum) for 45 mintes at 20°C.  An HRP-conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Rhein VF  et al. Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase. FEBS Lett 591:1641-1652 (2017). Read more (PubMed: 28391595) »
  • Li LX  et al. Lysine methyltransferase SMYD2 promotes cyst growth in autosomal dominant polycystic kidney disease. J Clin Invest 127:2751-2764 (2017). Read more (PubMed: 28604386) »

See all 19 Publications for this product

Publishing research using ab23366? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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24 reviews or Q&A

10
Votes: Highest First

Immunoprecipitation abreview for Anti-Methylated Lysine (di methyl , mono methyl ) antibody

 Below Average
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Hek293 - kidney cell line)
Total protein in input
1000 µg
Immuno-precipitation step
Other - Dynabeads or Protein G-coated magnetic beads
Specification
Hek293 - kidney cell line
Read More
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Username

Byron Baron

Verified customer

Submitted Sep 06 2018

Western blot abreview for Anti-Methylated Lysine (di methyl) antibody

 Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Hep3B)
Gel Running Conditions
Non-reduced Denaturing (15%)
Loading amount
20 µg
Specification
Hep3B
Blocking step
I-Block(Applied biosystems) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 23°C
Read More
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Username

Abcam user community

Verified customer

Submitted Jan 03 2017

Immunoprecipitation abreview for Anti-Methylated Lysine (di methyl , mono methyl ) antibody

 Good
Abreviews
Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (Human Embryonic Kidney 293 cells)
Specification
Human Embryonic Kidney 293 cells
Total protein in input
1000 µg
Read More
abreview image
Username

Dr. Yi-Wen Chang

Verified customer

Submitted Jan 26 2015

I ordered the Anti Methylated Lysine antibody (ab23366), but the product size is not listed. Do you mind telling me the molecular weight.


The short answer is that there is no predicted MW for this antibody to detect in WB. This antibody will detect any protein of any size that has a methylated lysine. Therefore, depending on your sample you may see no bands, many bands, a smear, e...

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I need Discount code for the three below antibodies . Please arrange to send the same at your earliest .

ab21623, ab23366 and ab412

for detection of methylation and acetylation of bacterial proteins.

Regards

Thank you for contacting us.

These antibodies are raised against targets that are not species specific. The antibodies would detect the proteins irrespective of species so the Abtrial discount will not be valid.

I hope this informatio...

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I noticed that Abcam has in its inventory anti mono- and di-methyl arginine antibody (ab412), and anti-methylated lysine (mono-methyl, dimethyl) antibody (ab23366).
Could you please send us a small sample of the antibodies mentioned above for a te...

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Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

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Thanks for this information. May I ask two more questions?
How much is the discrimination factor against BSA alone and is this antibody specific to methyllysine, or may it crossreact with methylarginine as well?
Thanks you very much for your ...

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Thank you for your reply.

I can confirm that ab23366 does not recognize free BSA or BSA-linked with methylarginine.

I hope this information is helpful and wish you good luck with your experiments.

Product code: 23366
Lot number: GR32966-7

Inquiry: Dear abcam-customer service! I actually do not really have a problem with the above-mentioned product, rather a question regarding its specificity. Actually, in a recent publication (Le...

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Thank you for your inquiry.

I apologize for the delay in my answer. It took some time to pull the data for lot GR32966-7.

I am happy to confirm that we tested lot GR32966-7 not in a peptide array buton the single amino acid linked t...

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Thank you very much for your kind replying and help. I am going to place a order for our substrate specificity studies. However, There are four anti-lysine abs available in Abcam as shown thehere. any difference among these Abs?Which one is the best fo...

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Thank you for your reply.

Based on the available information, any of those antibodies would work for you, but I would recommend ab21623.

If there is anything else I can help you with, please let me know.

We would like to order two antibodies with Anti-Methylated Lysine (mono methyl, di methyl) antibody (ab23366) and Anti-acetyl Lysine antibody - ChIP Grade (ab21623). Before the order, I have a few questions would like to ask.



1. ...

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Thank you for contacting Abcam.

Please find the answers to your question below:

1 - For ab21623, it will recognize proteins acetylated on lysine residues. For ab23366, it will recognize proteins methylated on lysine residues (mono- ...

Read More

1-10 of 24 Abreviews or Q&A

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