Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)
Key features and details
- Rabbit polyclonal to Methylated Lysine (di methyl , mono methyl )
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Methylated Lysine (di methyl , mono methyl ) antibody
See all Methylated Lysine primary antibodies -
Description
Rabbit polyclonal to Methylated Lysine (di methyl , mono methyl ) -
Host species
Rabbit -
Specificity
Recognize proteins methylated on lysine residues (mono- & di-methyl lysine). This antibody was affinity purified with mono-methyl lysine and di-methyl lysine, and may also react with tri-methyl lysine. Does not cross-react with acetylated protein. -
Tested applications
Suitable for: IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Methylated Lysine conjugated to KLH.
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Positive control
- HCL (0.1N) extracted and acetone precipited histone fraction
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 6.00
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified using N-methyl (epsilon amino group)lysine on Agarose as affinity matrix. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (3) |
Use at an assay dependent concentration.
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WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Do not use milk for any of the buffers as complex milk proteins may contain competitive methylated proteins. Use BSA instead. ab23366 incubation time 2h RT. If no or weak signal is obtained overnight at 4deg might help.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 1 µg/ml. Do not use milk for any of the buffers as complex milk proteins may contain competitive methylated proteins. Use BSA instead. ab23366 incubation time 2h RT. If no or weak signal is obtained overnight at 4deg might help. |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Many proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains. -
Alternative names
- Dimethylysine antibody
- Methyl lysine antibody
- N epsilon dimethyl lysine antibody
see all
Images
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Lane 1 : Anti-Methylated Lysine (tri methyl) antibody (does not cross react with mono, or di methyl lysine residues)
Lane 2 : Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)
All lanes : Yeast extract -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)This image is courtesy of an anonymous Abreviewab23366 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed a heat mediated antigen retrieval step was performed in citrate buffer. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)This image is courtesy of an anonymous Abreview
ab23366 staining formalin fixed paraffin-embedded rat kidney tissue sections. The section was subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 5% serum for 30 minutes at 20°C, prior to incubation with the primary antibody (diluted 1/200 in PBS + 5% serum) for 45 mintes at 20°C. An HRP-conjugated goat anti-rabbit antibody was used as the secondary.
Protocols
References (27)
ab23366 has been referenced in 27 publications.
- Wang T et al. SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism. Mol Cell 75:823-834.e5 (2019). PubMed: 31302001
- Malecki JM et al. Human FAM173A is a mitochondrial lysine-specific methyltransferase that targets adenine nucleotide translocase and affects mitochondrial respiration. J Biol Chem 294:11654-11664 (2019). PubMed: 31213526
- Feldman M & Levy D Peptide inhibition of the SETD6 methyltransferase catalytic activity. Oncotarget 9:4875-4885 (2018). PubMed: 29435148
- Bao L et al. Methylation of hypoxia-inducible factor (HIF)-1a by G9a/GLP inhibits HIF-1 transcriptional activity and cell migration. Nucleic Acids Res 46:6576-6591 (2018). PubMed: 29860315
- Yaseen I et al. Histone methyltransferase SUV39H1 participates in host defense by methylating mycobacterial histone-like protein HupB. EMBO J 37:183-200 (2018). PubMed: 29170282