Overview

  • Product name

    Methylglyoxal Assay Kit
    See all Methylglyoxal kits
  • Detection method

    Colorimetric
  • Product overview

    The Methylglyoxal assay kit (ab241006) enables the detection of MG using a set of engineered enzymes and a chromophore. The reduced chromophore, final product of the assay, produces a stable signal, which can be easily quantified at 450 nm using a microplate reader and its signal is directly proportional to the amount of MG in samples.


    The assay is simple, specific, reproducible, and can detect as low as 0.5 nmol/well of MG in a 100 μL reaction.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Enzyme Mix A 1 x 22µl
    Enzyme Mix B 1 x 120µl
    Enzyme Mix C 1 vial
    MG Assay Buffer 1 x 25ml
    MG Standard (20 mM) 1 x 1.1ml
    Substrate Mix A 1 vial
    Substrate Mix B 1 vial
  • Research areas

  • Relevance

    Methylglyoxal, also called pyruvaldehyde or 2-oxo-propanal (CH3-CO-CH=O or C3H4O2) is the aldehyde form of pyruvic acid. Methylglyoxal is both an aldehyde and a ketone. In the body, methylglyoxal may form from 3-amino acetone, which is an intermediate of threonine catabolism. This compound, as well as glyoxal, is a byproduct of metabolism, but cannot be used. The aldehyde reacts with glutathione, a tripeptide consisting of glutamic acid, cysteine, and glycine. Glutathione reduces methylglyoxal to 1-hydroxy acetone, a ketol. 1-Hydroxy acetone goes through enediol tautomerism to lactaldehyde. Then the lactaldehyde is oxidised by glutathione to D-lactic acid. Methylglyoxal is also called a ketal, because it has an aldehydic and ketonic carbonyl group. It is a type of the advanced glycation endproducts (AGEs) resulting from sugar modifications, such as the Maillard reaction. When the reductone is cleaved, pyruvaldehyde forms.
  • Alternative names

    • 2 oxo propanal
    • MG
    • pyruvaldehyde

Images

  • MG Standard Curve.

  • Measurement of MG in Manuka Honey (MGO 30+) (10 µl; Dilution Factor: 2, in dH2O); Manuka Honey (UMF 15+) (10 µl; Dilution Factor: 10, in dH2O). All assays were performed following kit protocols. *According to reference: MG in Manuka Honey (MGO 30+): ≥ 30 mg/kg; MG in Manuka Honey (UMF 15+): ≥ 510 mg/kg.

Protocols

References

ab241006 has not yet been referenced specifically in any publications.

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