Product nameMethylglyoxal ELISA Kit
See all Methylglyoxal kits
Sample typeSerum, Plasma, Cell Lysate, Purified protein, Tissue Lysate
Range0.2 µg/ml - 25 µg/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Vertebrata
Methylglyoxal ELISA Kit (ab238543) is developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts.
The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.
Tested applicationsSuitable for: Competitive ELISAmore details
Storage instructionsPlease refer to protocols.
Components 96 tests 100X Conjugate Diluent 1 x 300µl 10X Wash Buffer 1 x 100ml Protein Binding Strip Well Plate 1 unit Anti-MG Antibody (1000X) 1 x 10µl Assay Diluent 1 x 50ml MG Conjugate 1 x 20µl MG-BSA Standard 1 x 75µl Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl Stop Solution 1 x 12ml Substrate Solution 1 x 12ml
RelevanceMethylglyoxal, also called pyruvaldehyde or 2-oxo-propanal (CH3-CO-CH=O or C3H4O2) is the aldehyde form of pyruvic acid. Methylglyoxal is both an aldehyde and a ketone. In the body, methylglyoxal may form from 3-amino acetone, which is an intermediate of threonine catabolism. This compound, as well as glyoxal, is a byproduct of metabolism, but cannot be used. The aldehyde reacts with glutathione, a tripeptide consisting of glutamic acid, cysteine, and glycine. Glutathione reduces methylglyoxal to 1-hydroxy acetone, a ketol. 1-Hydroxy acetone goes through enediol tautomerism to lactaldehyde. Then the lactaldehyde is oxidised by glutathione to D-lactic acid. Methylglyoxal is also called a ketal, because it has an aldehydic and ketonic carbonyl group. It is a type of the advanced glycation endproducts (AGEs) resulting from sugar modifications, such as the Maillard reaction. When the reductone is cleaved, pyruvaldehyde forms.
- 2 oxo propanal
Our Abpromise guarantee covers the use of ab238543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
ab238543 has not yet been referenced specifically in any publications.