Overview

  • Product name

    Methylglyoxal ELISA Kit
    See all Methylglyoxal kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Cell Lysate, Purified protein, Tissue Lysate
  • Assay type

    Competitive
  • Range

    0.2 µg/ml - 25 µg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Vertebrata
  • Product overview

    Methylglyoxal ELISA Kit (ab238543) is developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts.


    The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 96 tests
    100X Conjugate Diluent 1 x 300µl
    10X Wash Buffer 1 x 100ml
    Protein Binding Strip Well Plate 1 unit
    Anti-MG Antibody (1000X) 1 x 10µl
    Assay Diluent 1 x 50ml
    MG Conjugate 1 x 20µl
    MG-BSA Standard 1 x 75µl
    Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl
    Stop Solution 1 x 12ml
    Substrate Solution 1 x 12ml
  • Research areas

  • Relevance

    Methylglyoxal, also called pyruvaldehyde or 2-oxo-propanal (CH3-CO-CH=O or C3H4O2) is the aldehyde form of pyruvic acid. Methylglyoxal is both an aldehyde and a ketone. In the body, methylglyoxal may form from 3-amino acetone, which is an intermediate of threonine catabolism. This compound, as well as glyoxal, is a byproduct of metabolism, but cannot be used. The aldehyde reacts with glutathione, a tripeptide consisting of glutamic acid, cysteine, and glycine. Glutathione reduces methylglyoxal to 1-hydroxy acetone, a ketol. 1-Hydroxy acetone goes through enediol tautomerism to lactaldehyde. Then the lactaldehyde is oxidised by glutathione to D-lactic acid. Methylglyoxal is also called a ketal, because it has an aldehydic and ketonic carbonyl group. It is a type of the advanced glycation endproducts (AGEs) resulting from sugar modifications, such as the Maillard reaction. When the reductone is cleaved, pyruvaldehyde forms.
  • Alternative names

    • 2 oxo propanal
    • MG
    • pyruvaldehyde

Applications

Our Abpromise guarantee covers the use of ab238543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Typical Methylglyoxal (MG) Competitive ELISA results.

    This data is for reference only and should not be used to interpret actual results.

Protocols

References

ab238543 has not yet been referenced specifically in any publications.

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