Overview

  • Product name

    Anti-Methylmalonyl Coenzyme A mutase antibody
    See all Methylmalonyl Coenzyme A mutase primary antibodies
  • Description

    Rabbit polyclonal to Methylmalonyl Coenzyme A mutase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Methylmalonyl Coenzyme A mutase (internal sequence). The exact sequence is proprietary.
    Database link: P22033

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab229486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.

Target

  • Function

    Involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.
  • Involvement in disease

    Defects in MUT are the cause of methylmalonic aciduria type mut (MMAM) [MIM:251000]. MMAM is an often fatal disorder of organic acid metabolism. Common clinical features include lethargy, vomiting, failure to thrive, hypotonia, neurological deficit and early death. Two forms of the disease are distinguished by the presence (mut-) or absence (mut0) of residual enzyme activity. Mut0 patients have more severe neurological manifestations of the disease than do MUT- patients. MMAM is unresponsive to vitamin B12 therapy.
  • Sequence similarities

    Belongs to the methylmalonyl-CoA mutase family.
    Contains 1 B12-binding domain.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • MCM antibody
    • Methylmalonyl CoA isomerase antibody
    • Methylmalonyl CoA mutase mitochondrial antibody
    • Methylmalonyl Coenzyme A mutase antibody
    • Methylmalonyl-CoA isomerase antibody
    • Methylmalonyl-CoA mutase antibody
    • mitochondrial antibody
    • Mut antibody
    • MUTA_HUMAN antibody
    see all

Images

  • All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody (ab229486) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 83 kDa



    7.5% SDS-PAGE gel.

References

ab229486 has not yet been referenced specifically in any publications.

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