Key features and details
- Rabbit polyclonal to Methylmalonyl Coenzyme A mutase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Methylmalonyl Coenzyme A mutase antibody
See all Methylmalonyl Coenzyme A mutase primary antibodies
DescriptionRabbit polyclonal to Methylmalonyl Coenzyme A mutase
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Rhesus monkey
Recombinant fragment within Human Methylmalonyl Coenzyme A mutase (internal sequence). The exact sequence is proprietary.
Database link: P22033
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 83 kDa.|
FunctionInvolved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.
Involvement in diseaseDefects in MUT are the cause of methylmalonic aciduria type mut (MMAM) [MIM:251000]. MMAM is an often fatal disorder of organic acid metabolism. Common clinical features include lethargy, vomiting, failure to thrive, hypotonia, neurological deficit and early death. Two forms of the disease are distinguished by the presence (mut-) or absence (mut0) of residual enzyme activity. Mut0 patients have more severe neurological manifestations of the disease than do MUT- patients. MMAM is unresponsive to vitamin B12 therapy.
Sequence similaritiesBelongs to the methylmalonyl-CoA mutase family.
Contains 1 B12-binding domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- MCM antibody
- Methylmalonyl CoA isomerase antibody
- Methylmalonyl CoA mutase mitochondrial antibody
All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody (ab229486) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 83 kDa
7.5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229486 has not yet been referenced specifically in any publications.