Overview

  • Product name

    Anti-Methylmalonyl Coenzyme A mutase antibody
    See all Methylmalonyl Coenzyme A mutase primary antibodies
  • Description

    Mouse polyclonal to Methylmalonyl Coenzyme A mutase
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Full length Methylmalonyl Coenzyme A mutase protein (Human)

  • Positive control

    • Methylmalonyl Coenzyme A mutase transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IHC-P Use at an assay dependent dilution. PubMed: 19699272

Target

  • Function

    Involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.
  • Involvement in disease

    Defects in MUT are the cause of methylmalonic aciduria type mut (MMAM) [MIM:251000]. MMAM is an often fatal disorder of organic acid metabolism. Common clinical features include lethargy, vomiting, failure to thrive, hypotonia, neurological deficit and early death. Two forms of the disease are distinguished by the presence (mut-) or absence (mut0) of residual enzyme activity. Mut0 patients have more severe neurological manifestations of the disease than do MUT- patients. MMAM is unresponsive to vitamin B12 therapy.
  • Sequence similarities

    Belongs to the methylmalonyl-CoA mutase family.
    Contains 1 B12-binding domain.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • MCM antibody
    • Methylmalonyl CoA isomerase antibody
    • Methylmalonyl CoA mutase mitochondrial antibody
    • Methylmalonyl Coenzyme A mutase antibody
    • Methylmalonyl-CoA isomerase antibody
    • Methylmalonyl-CoA mutase antibody
    • mitochondrial antibody
    • Mut antibody
    • MUTA_HUMAN antibody
    see all

Images

  • Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/500 dilution + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

  • All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/500 dilution

    Lane 1 : Methylmalonyl Coenzyme A mutase transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

  • ICC/IF image of ab67869 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67869, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/2000 dilution

    Lane 1 : Euglena whole cell lysate with Milk and 0.05% Tween20
    Lane 2 : Euglena (RNAi gene silencing Methylmalonyl Coenzyme A mutase) whole cell lysate, with Milk and 0.05% Tween20

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG(H+L) HRP conjugated at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 83 kDa


    Exposure time: 10 minutes

    See Abreview

References

This product has been referenced in:

  • Forny P  et al. Novel Mouse Models of Methylmalonic Aciduria Recapitulate Phenotypic Traits with a Genetic Dosage Effect. J Biol Chem 291:20563-73 (2016). Read more (PubMed: 27519416) »
  • Ruppert T  et al. Molecular and biochemical alterations in tubular epithelial cells of patients with isolated methylmalonic aciduria. Hum Mol Genet 24:7049-59 (2015). Read more (PubMed: 26420839) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Euglena Cell lysate - whole cell (Euglena gracilis SM-ZK)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Treatment
Gene silencing
Specification
Euglena gracilis SM-ZK
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

昌美 中澤

Verified customer

Submitted Oct 31 2017

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for ICC/IF, WB, IHC-P and in rat and human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

Regrettably, as discussed on the telephone, ab67869 has not been not tested mouse or IHC-Fr (frozen sections). Therefore, we are not able to provide any replacement on this occasion. I have checked the alignment of the human protein with the mouse, and this is quite high at 95%. However, unfortunately there are other factors that influence how well an antibody will work in a different species and so we are not able to guarantee this without further testing.

I can highly recommend to consider trying it in a tested application with a positive control sample from human or rat, this will be covered by the guarantee.

The other Methylmalonyl Coenzyme A mutase we have that are tested in mouse are ab133672, ab134956. I am sorry these have not been tested in IHC-Fr. However, they have been tested in IHC-P.

These are both from our RabMab range. As discussed on the telephone, we have a special offer on at the moment to receive on of our RabMab antibodies free when you purchase another primary antibody. The details of the offer are on the following page from our website:

https://www.abcam.com/index.html?pageconfig=resource&rid=15447


Regarding the results from ab67869, I would be pleased to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.

Order Details
Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results

Read More

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