For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    methylmalonyl-coenzyme-a-mutase-antibody-epr7739-ab133672.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Amino Acids
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7739] to Methylmalonyl Coenzyme A mutase
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human Methylmalonyl Coenzyme A mutase protein (ab114834)
Reagent
Product image
VeriBlot for IP Detection Reagent (HRP) (ab131366)
Biochemical
Product image
AEC (3-Amino-9-ethylcarbazole), Peroxidase substrate (ab142228)

View more associated products

Overview

  • Product name

    Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739]
    See all Methylmalonyl Coenzyme A mutase primary antibodies
  • Description

    Rabbit monoclonal [EPR7739] to Methylmalonyl Coenzyme A mutase
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Methylmalonyl Coenzyme A mutase aa 550-650. The exact sequence is proprietary.
    Database link: P22033

  • Positive control

    • WB: NIH 3T3, K562, 293T, HEK-293, PC-12, C6, mouse kidney, rat kidney, human fetal liver and HeLa cell lysates IHC-P: Human kidney and livertissue ICC/IF: HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 9.40 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7739
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] - BSA and Azide free (ab236068)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Methylmalonyl Coenzyme A mutase protein (ab114834)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 83 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

ICC/IF
1/100 - 1/250.
Notes
WB
1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 83 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.
  • Involvement in disease

    Defects in MUT are the cause of methylmalonic aciduria type mut (MMAM) [MIM:251000]. MMAM is an often fatal disorder of organic acid metabolism. Common clinical features include lethargy, vomiting, failure to thrive, hypotonia, neurological deficit and early death. Two forms of the disease are distinguished by the presence (mut-) or absence (mut0) of residual enzyme activity. Mut0 patients have more severe neurological manifestations of the disease than do MUT- patients. MMAM is unresponsive to vitamin B12 therapy.
  • Sequence similarities

    Belongs to the methylmalonyl-CoA mutase family.
    Contains 1 B12-binding domain.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P22033 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4594 Human
    • Entrez Gene: 17850 Mouse
    • Entrez Gene: 688517 Rat
    • Omim: 609058 Human
    • SwissProt: P22033 Human
    • SwissProt: P16332 Mouse
    • Unigene: 485527 Human
    • Unigene: 259884 Mouse
    • Alternative names

      • MCM antibody
      • Methylmalonyl CoA isomerase antibody
      • Methylmalonyl CoA mutase mitochondrial antibody
      • Methylmalonyl Coenzyme A mutase antibody
      • Methylmalonyl-CoA isomerase antibody
      • Methylmalonyl-CoA mutase antibody
      • mitochondrial antibody
      • Mut antibody
      • MUTA_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Methylmalonyl Coenzyme A mutase with Purified ab133672 at 1:100 dilution (8.7 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
    • Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Methylmalonyl Coenzyme A mutase with Purified ab133672 at 1:100 dilution (9.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672) at 0.9 µg/ml (purified)

      Lane 1 : Mouse kidney lysates
      Lane 2 : Rat kidney lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 83 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672) at 0.9 µg/ml (purified)

      Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
      Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
      Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 83 kDa



      Blocking and diluting buffer: 5% NFDM/TBST. 

    • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Methylmalonyl Coenzyme A mutase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab133672 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.

      Unpurified ab133672 was shown to specifically react with Methylmalonyl Coenzyme A mutase when Methylmalonyl Coenzyme A mutase knockout samples were used. Wild-type and Methylmalonyl Coenzyme A mutase knockout samples were subjected to SDS-PAGE. ab133672 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Methylmalonyl Coenzyme A mutase with unpurified ab133672 at 1/50.

    • Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)

      Immunofluorescent staining of HeLa cells labelling Methylmalonyl Coenzyme A mutase with unpurified ab133672 at 1/100.

    • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672) at 1/1000 dilution (Unpurified)

      Lane 1 : NIH 3T3 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 83 kDa

    • Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab133672? Please let us know so that we can cite the reference in this datasheet.

    ab133672 has been referenced in 1 publication.

    • Dalton WB  et al. Hotspot SF3B1 mutations induce metabolic reprogramming and vulnerability to serine deprivation. J Clin Invest 129:4708-4723 (2019). PubMed: 31393856

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Heart (Embryonic))
    Permeabilization
    Yes - Tween-20
    Specification
    Heart (Embryonic)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 29 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.