Key features and details
- Rabbit polyclonal to Metnase
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Metnase antibody
See all Metnase primary antibodies
DescriptionRabbit polyclonal to Metnase
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Gorilla, Orangutan
Synthetic peptide corresponding to Human Metnase aa 1-50 (N terminal). (BAD96454.1)
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
Previously labelled as SETMAR.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab129455 is purified using an epitope specific to Metnase immobilized on solid support.
Our Abpromise guarantee covers the use of ab129455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 77 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionHistone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3, 2 specific tags for epigenetic transcriptional activation. Specifically mediates dimethylation of H3 'Lys-36'. Has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. Has DNA nicking activity. Has in vivo end joining activity and may mediate genomic integration of foreign DNA.
Tissue specificityWidely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle.
Sequence similaritiesIn the N-terminal section; belongs to the histone-lysine methyltransferase family.
In the C-terminal section; belongs to the mariner transposase family.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
DomainThe mariner transposase Hsmar1 region mediates DNA-binding. It has no transposase activity because the active site contains an Asn in position 610 instead of a Asp residue.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- Histone lysine N methyltransferase antibody
- Histone lysine N methyltransferase SETMAR antibody
- Hsmar 1 antibody
All lanes : Anti-Metnase antibody (ab129455) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 30 seconds
Detection of Metnase by Western Blot of Immunoprecipitates.
ab129455 at 1µg/ml staining Metnase in HeLa whole cell lysate immunoprecipitated using ab129455 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab129455 has been referenced in 2 publications.
- Dussaussois-Montagne A et al. SETMAR isoforms in glioblastoma: A matter of protein stability. Oncotarget 8:9835-9848 (2017). PubMed: 28038463
- Carlson SM et al. A Proteomic Strategy Identifies Lysine Methylation of Splicing Factor snRNP70 by the SETMAR Enzyme. J Biol Chem 290:12040-7 (2015). PubMed: 25795785