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    metrsmars-antibody-epr9872-ab180497.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs
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RecombinantRabMAb

Recombinant Anti-MetRS/MARS antibody [EPR9872] (ab180497)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
  • Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
  • Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
  • Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
  • Immunoprecipitation - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
  • Flow Cytometry (Intracellular) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
  • Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
  • Immunoprecipitation - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
  • Anti-MetRS/MARS antibody [EPR9872] (ab180497)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9872] to MetRS/MARS
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, IP, ICC/IF
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-MetRS/MARS antibody [EPR9872]
    See all MetRS/MARS primary antibodies
  • Description

    Rabbit monoclonal [EPR9872] to MetRS/MARS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human MetRS/MARS aa 150-250. The exact sequence is proprietary.
    Database link: P56192

  • Positive control

    • WB: HeLa, HepG2, SW480, Caco2 and PC-12 lysates. IHC-P: human thyroid carcinoma and brain tissues, and rat kidney tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9872
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNA synthetase

Associated products

  • Alternative Versions

    • Anti-MetRS/MARS antibody [EPR9872] - BSA and Azide free (ab250200)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • SW480 whole cell lysate (ab3957)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/50

See IHC antigen retrieval protocols.

WB
1/10000 - 1/50000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
IP
1/20 - 1/40.
ICC/IF
1/50 - 1/250.
Notes
Flow Cyt (Intra)
1/20.
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/50

See IHC antigen retrieval protocols.

WB
1/10000 - 1/50000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
IP
1/20 - 1/40.
ICC/IF
1/50 - 1/250.

Target

  • Sequence similarities

    Belongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 GST C-terminal domain.
    Contains 1 WHEP-TRS domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P56192 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4141 Human
    • Entrez Gene: 299851 Rat
    • Omim: 156560 Human
    • SwissProt: P56192 Human
    • Unigene: 632707 Human
    • Alternative names

      • cytoplasmic antibody
      • FLJ35667 antibody
      • Mars antibody
      • Methionine tRNA ligase 1 antibody
      • Methionine tRNA ligase 1 cytoplasmic antibody
      • Methionine tRNA ligase antibody
      • Methionine tRNA synthetase antibody
      • Methionine--tRNA ligase antibody
      • Methionyl tRNA synthetase cytoplasmic antibody
      • Methionyl-tRNA synthetase antibody
      • MetRS antibody
      • MRS antibody
      • MTRNS antibody
      • SYMC_HUMAN antibody
      see all

    Images

    • Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      All lanes : Anti-MetRS/MARS antibody [EPR9872] (ab180497) at 1/10000 dilution (Purified)

      Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 15 µg


      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 101 kDa
      Observed band size: 101 kDa

    • Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      All lanes : Anti-MetRS/MARS antibody [EPR9872] (ab180497) at 1/50000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 101 kDa
      Observed band size: 101 kDa

    • Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      All lanes : Anti-MetRS/MARS antibody [EPR9872] (ab180497) at 1/10000 dilution ((unpurified))

      Lane 1 : HepG2 lysate
      Lane 2 : HeLa lysate
      Lane 3 : SW480 lysate
      Lane 4 : Caco2 lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 101 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MetRS/MARS with purified ab180497 at 1/50 dilution (4.52 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling MetRS/MARS with purified ab180497 at 1/4000 dilution (0.06 µg/ml). Heat mediated antigen retrieval using BondTM Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunoprecipitation - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Immunoprecipitation - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

      ab180497 (purified ) at 1/20 dilution (1ug) immunoprecipitating MetRS/MARS in HepG2 whole cell lysate.
      Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10ug
      Lane 2 (+): ab180497 & HepG2 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180497 in HepG2 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Flow Cytometry (Intracellular) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

      Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MetRS/MARS with purified ab180497 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling MetRS/MARS with purified ab180497 at 1/4000 dilution (0.06 µg/ml). Heat mediated antigen retrieval using BondTM Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

      Immunofluorescent analysis of HeLa cells (acetone-fixed) labeling MARS with unpurified ab180497 at 1/250 dilution (green) followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution and DAPI staining (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling MARS with unpurified ab180497 at 1/50 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Immunoprecipitation - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

      Western blot analysis of immunoprecipitation pellet from HepG2 lysate immunoprecipitated using unpurified ab180497 at 1/40 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

    • Anti-MetRS/MARS antibody [EPR9872] (ab180497)
      Anti-MetRS/MARS antibody [EPR9872] (ab180497)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (2)

    Publishing research using ab180497? Please let us know so that we can cite the reference in this datasheet.

    ab180497 has been referenced in 2 publications.

    • Hei Z  et al. Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex. J Biol Chem 294:4775-4783 (2019). PubMed: 30733335
    • Abuduxikuer K  et al. Novel methionyl-tRNA synthetase gene variants/phenotypes in interstitial lung and liver disease: A case report and review of literature. World J Gastroenterol 24:4208-4216 (2018). PubMed: 30271085

    Customer reviews and Q&As

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