Recombinant Anti-MetRS/MARS antibody [EPR9872] (ab180497)

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Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9872] to MetRS/MARS
Suitable for: Flow Cyt (Intra), IHC-P, WB, IP, ICC/IF
Reacts with: Rat, Human

Carrier Free

Product name

Anti-MetRS/MARS antibody [EPR9872]
See all MetRS/MARS primary antibodies
Description

Rabbit monoclonal [EPR9872] to MetRS/MARS
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), IHC-P, WB, IP, ICC/IFmore details
Species reactivity

Reacts with: Rat, Human
Immunogen

Synthetic peptide within Human MetRS/MARS aa 150-250. The exact sequence is proprietary.
Database link: P56192
Positive control
- WB: HeLa, HepG2, SW480, Caco2 and PC-12 lysates. IHC-P: human thyroid carcinoma and brain tissues, and rat kidney tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9872
Isotype

IgG
Research areas

Alternative Versions
- Anti-MetRS/MARS antibody [EPR9872] - BSA and Azide free (ab250200)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/20.
IHC-P		1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/50 See IHC antigen retrieval protocols.
WB		1/10000 - 1/50000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
IP		1/20 - 1/40.
ICC/IF		1/50 - 1/250.

Notes
Flow Cyt (Intra) 1/20.
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/50 See IHC antigen retrieval protocols.
WB 1/10000 - 1/50000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
IP 1/20 - 1/40.
ICC/IF 1/50 - 1/250.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 GST C-terminal domain.
Contains 1 WHEP-TRS domain.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession P56192 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4141 Human
- Entrez Gene: 299851 Rat
- Omim: 156560 Human
- SwissProt: P56192 Human
- Unigene: 632707 Human
Alternative names
- cytoplasmic antibody
- FLJ35667 antibody
- Mars antibody
- Methionine tRNA ligase 1 antibody
- Methionine tRNA ligase 1 cytoplasmic antibody
- Methionine tRNA ligase antibody
- Methionine tRNA synthetase antibody
- Methionine--tRNA ligase antibody
- Methionyl tRNA synthetase cytoplasmic antibody
- Methionyl-tRNA synthetase antibody
- MetRS antibody
- MRS antibody
- MTRNS antibody
- SYMC_HUMAN antibody
see all

Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

All lanes : Anti-MetRS/MARS antibody [EPR9872] (ab180497) at 1/10000 dilution (Purified)

Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 15 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 101 kDa
Observed band size: 101 kDa
Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

All lanes : Anti-MetRS/MARS antibody [EPR9872] (ab180497) at 1/50000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 101 kDa
Observed band size: 101 kDa
Western blot - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

All lanes : Anti-MetRS/MARS antibody [EPR9872] (ab180497) at 1/10000 dilution ((unpurified))

Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : SW480 lysate
Lane 4 : Caco2 lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 101 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MetRS/MARS with purified ab180497 at 1/50 dilution (4.52 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling MetRS/MARS with purified ab180497 at 1/4000 dilution (0.06 µg/ml). Heat mediated antigen retrieval using Bond^TM Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunoprecipitation - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

ab180497 (purified ) at 1/20 dilution (1ug) immunoprecipitating MetRS/MARS in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab180497 & HepG2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180497 in HepG2 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry (Intracellular) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MetRS/MARS with purified ab180497 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling MetRS/MARS with purified ab180497 at 1/4000 dilution (0.06 µg/ml). Heat mediated antigen retrieval using Bond^TM Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

Immunofluorescent analysis of HeLa cells (acetone-fixed) labeling MARS with unpurified ab180497 at 1/250 dilution (green) followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution and DAPI staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling MARS with unpurified ab180497 at 1/50 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-MetRS/MARS antibody [EPR9872] (ab180497)

Western blot analysis of immunoprecipitation pellet from HepG2 lysate immunoprecipitated using unpurified ab180497 at 1/40 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Anti-MetRS/MARS antibody [EPR9872] (ab180497)

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab180497? Please let us know so that we can cite the reference in this datasheet.

ab180497 has been referenced in 2 publications.

Hei Z et al. Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex. J Biol Chem 294:4775-4783 (2019). PubMed: 30733335
Abuduxikuer K et al. Novel methionyl-tRNA synthetase gene variants/phenotypes in interstitial lung and liver disease: A case report and review of literature. World J Gastroenterol 24:4208-4216 (2018). PubMed: 30271085

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (2)

Customer reviews and Q&As