Recombinant Anti-MetRS/MARS antibody [EPR9873(B)] (ab137105)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9873(B)] to MetRS/MARS
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MetRS/MARS antibody [EPR9873(B)]
See all MetRS/MARS primary antibodies -
Description
Rabbit monoclonal [EPR9873(B)] to MetRS/MARS -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MetRS/MARS aa 800-900. The exact sequence is proprietary.
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Positive control
- HepG2, HeLa, SW480 and Caco-2 cell lysates; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9873(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/250 - 1/500.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 101 kDa.
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Notes |
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ICC/IF
1/250 - 1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 101 kDa. |
Target
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Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 GST C-terminal domain.
Contains 1 WHEP-TRS domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4141 Human
- Omim: 156560 Human
- SwissProt: P56192 Human
- Unigene: 632707 Human
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Alternative names
- cytoplasmic antibody
- FLJ35667 antibody
- Mars antibody
see all
Images
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All lanes : Anti-MetRS/MARS antibody [EPR9873(B)] (ab137105) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 101 kDa -
Immunofluorescent analysis of HepG2 cells labelling MetRS/MARS with ab137105 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab137105 has been referenced in 1 publication.
- Lee K et al. Is Methionyl-tRNA Synthetase Applicable as a Diagnostic Marker for Lung Cancer in Bronchial Ultrasound-Guided Brushing Cells? Diagnostics (Basel) 11:N/A (2021). PubMed: 34679529