Overview

  • Product name

    Anti-MetRS/MARS antibody [MARSD10B4]
    See all MetRS/MARS primary antibodies
  • Description

    Mouse monoclonal [MARSD10B4] to MetRS/MARS
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human MetRS/MARS aa 50-200 (C terminal).

  • Positive control

    • WB: NIH3T3 whole cell, HeLa whole cell and F2408 whole cell lysates. IP: RIPA lysate of HeLa cells. ICC: HeLa cell. FC: Hela cells.
  • General notes

     This product was previously labelled as Methionyl tRNA synthetase, MARS

     

Properties

Applications

Our Abpromise guarantee covers the use of ab50793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
Flow Cyt 1/10.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/10.
IP Use at 5 µg/mg of lysate.

Target

Images

  • Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 101 kDa
    Observed band size: 98 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation from 200ug RIPA lysate of HeLa cells using 5ug of ab50793. Visualized by Western Blot using ab50793 at 1/100 dilution, anti-mouse IgG secondary 1/1000 and ECL detection.

    Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 5 µg + RIPA lysate of HeLa cells at 200 µg

    Observed band size: 110 kDa why is the actual band size different from the predicted?

  • Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 101 kDa
    Observed band size: 98 kDa why is the actual band size different from the predicted?

  • Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution + F2408 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody

    Predicted band size: 101 kDa
    Observed band size: 98 kDa why is the actual band size different from the predicted?

  • Ab50793, at a dilution of 1/10, staining MetRS/MARS in Hela cell by Immunocytochemistry/Immunofluorescence. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/200.

  • Ab50793, at a dilution of 1/10, staining MetRS/MARS in Hela cells by Flow Cytometry. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/400.

References

This product has been referenced in:

  • Silva RC  et al. A Rapid Extraction Method for mammalian cell cultures, suitable for quantitative immunoblotting analysis of proteins, including phosphorylated GCN2 and eIF2a. MethodsX 5:75-82 (2018). Read more (PubMed: 30619721) »
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »
See all 4 Publications for this product

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