Key features and details
- Rabbit polyclonal to METTL14
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-METTL14 antibody
See all METTL14 primary antibodies
DescriptionRabbit polyclonal to METTL14
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Orangutan
Recombinant fragment corresponding to Human METTL14 aa 24-206.
GAESADSIGAVLNSKDEQREIAETRETCRASYDTSAPNAKRKYLDEGETD EDKMEEYKDELEMQQDEENLPYEEEIYKDSSTFLKGTQSLNPHNDYCQHF VDTGHRPQNFIRDVGLADRFEEYPKLRELIRLKDELIAKSNTPPMYLQAD IEAFDIRELTPKFDVILLEPPLEEYYRETGITA
Database link: Q9HCE5
- WB: SH-SY5Y whole cell lysate. IHC-P: Human gastric cancer and testis tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab223090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 52 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionN6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization.
Sequence similaritiesBelongs to the MT-A70-like family.
- Information by UniProt
- KIAA1627 antibody
- MET14_HUMAN antibody
- Methyltransferase like 14 antibody
Anti-METTL14 antibody (ab223090) at 1/1000 dilution + SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Paraffin-embedded human gastric cancer tissue stained for METTL14 using ab223090 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for METTL14 using ab223090 at 1/100 dilution in immunohistochemical analysis.
ab223090 has not yet been referenced specifically in any publications.