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Anti-METTL14 antibody (ab264408)

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Immunoprecipitation - Anti-METTL14 antibody (ab264408)

    Key features and details

    • Rabbit polyclonal to METTL14
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-METTL14 antibody
      See all METTL14 primary antibodies
    • Description

      Rabbit polyclonal to METTL14
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      IP
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide within Human METTL14 aa 75-125. The exact sequence is proprietary.
      Database link: Q9HCE5

    • Positive control

      • IP: Jurkat whole cell lysate.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: Tris buffered saline, 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Other Antibodies
      • Other Antibodies

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Positive Controls

      • Jurkat whole cell lysate (ab30128)
      • Jurkat whole cell lysate (ab7899)
    • Related Products

      • Recombinant Human METTL14 protein (ab163640)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab264408 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IP
    Human
    All applications
    Orangutan
    Application Abreviews Notes
    IP
    Use at an assay dependent concentration.

    Use 15-20 µl/mg lysate.

    Notes
    IP
    Use at an assay dependent concentration.

    Use 15-20 µl/mg lysate.

    Target

    • Function

      N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization.
    • Sequence similarities

      Belongs to the MT-A70-like family.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9HCE5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 57721 Human
      • Omim: 616504 Human
      • SwissProt: Q9HCE5 Human
      • SwissProt: Q5R5N4 Orangutan
      • Unigene: 596872 Human
      • Unigene: 657806 Human
      • Alternative names

        • KIAA1627 antibody
        • MET14_HUMAN antibody
        • Methyltransferase like 14 antibody
        • Methyltransferase-like protein 14 antibody
        • METTL14 antibody
        • N6-adenosine-methyltransferase subunit METTL14 antibody
        • OTTHUMP00000219837 antibody
        see all

      Images

      • Immunoprecipitation - Anti-METTL14 antibody (ab264408)
        Immunoprecipitation - Anti-METTL14 antibody (ab264408)

        METTL14 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab252562 at 100 μL per reaction. Western blot was performed from the immunoprecipitate using ab252562 at 1/1000 dilution.

        Lane 1: ab252562 IP in Jurkat whole cell lysate.
        Lane 2: ab264408 IP in Jurkat whole cell lysate.
        Lane 3: Control IgG IP in Jurkat whole cell lysate.

        Detection: Chemiluminescence with exposure time of 3 seconds.

        Lysates prepared in NETN buffer.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab264408? Please let us know so that we can cite the reference in this datasheet.

      ab264408 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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