Product nameAnti-METTL14 antibody
See all METTL14 primary antibodies
DescriptionRabbit polyclonal to METTL14
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human METTL14 aa 75-125. The exact sequence is proprietary.
Database link: Q9HCE5
- IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 15-20 µl/mg lysate.
FunctionN6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization.
Sequence similaritiesBelongs to the MT-A70-like family.
- Information by UniProt
- KIAA1627 antibody
- MET14_HUMAN antibody
- Methyltransferase like 14 antibody
METTL14 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264408 at 15 µl per reaction. Western blot was performed from the immunoprecipitate using an alternative anti-METTL14 antibody.
Lane 1: ab264408 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Lysates prepared using NETN lysis buffer.
ab264408 has not yet been referenced specifically in any publications.