Anti-METTL14 antibody (ab264408)
Key features and details
- Rabbit polyclonal to METTL14
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-METTL14 antibody
See all METTL14 primary antibodies -
Description
Rabbit polyclonal to METTL14 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human METTL14 aa 75-125. The exact sequence is proprietary.
Database link: Q9HCE5 -
Positive control
- IP: Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| All applications |
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
Use 15-20 µl/mg lysate. |
| Notes |
|---|
|
IP
Use at an assay dependent concentration. Use 15-20 µl/mg lysate. |
Target
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Function
N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization. -
Sequence similarities
Belongs to the MT-A70-like family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57721 Human
- Omim: 616504 Human
- SwissProt: Q9HCE5 Human
- SwissProt: Q5R5N4 Orangutan
- Unigene: 596872 Human
- Unigene: 657806 Human
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Alternative names
- KIAA1627 antibody
- MET14_HUMAN antibody
- Methyltransferase like 14 antibody
see all
Images
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Immunoprecipitation - Anti-METTL14 antibody (ab264408)
METTL14 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab252562 at 100 μL per reaction. Western blot was performed from the immunoprecipitate using ab252562 at 1/1000 dilution.
Lane 1: ab252562 IP in Jurkat whole cell lysate.
Lane 2: ab264408 IP in Jurkat whole cell lysate.
Lane 3: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 3 seconds.
Lysates prepared in NETN buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab264408 has not yet been referenced specifically in any publications.
