Anti-METTL14 antibody (ab264408)

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Overview

  • Product name

    Anti-METTL14 antibody
    See all METTL14 primary antibodies

  • Description

    Rabbit polyclonal to METTL14

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan

  • Immunogen

    Synthetic peptide within Human METTL14 aa 75-125. The exact sequence is proprietary.
    Database link: Q9HCE5

  • Positive control

    • IP: Jurkat whole cell lysate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab264408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Use 15-20 µl/mg lysate.

Target

  • Function

    N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization.

  • Sequence similarities

    Belongs to the MT-A70-like family.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • KIAA1627 antibody
      • MET14_HUMAN antibody
      • Methyltransferase like 14 antibody
      • Methyltransferase-like protein 14 antibody
      • METTL14 antibody
      • N6-adenosine-methyltransferase subunit METTL14 antibody
      • OTTHUMP00000219837 antibody
      see all

    Images

    • Immunoprecipitation - Anti-METTL14 antibody (ab264408)
      Immunoprecipitation - Anti-METTL14 antibody (ab264408)

      METTL14 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264408 at 15 µl per reaction. Western blot was performed from the immunoprecipitate using an alternative anti-METTL14 antibody.

      Lane 1: ab264408 IP in Jurkat whole cell lysate.
      Lane 2: Control IgG IP in Jurkat whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 3 seconds.

      Lysates prepared using NETN lysis buffer.

    References

    ab264408 has not yet been referenced specifically in any publications.

    Publishing research using ab264408? Please let us know so that we can cite the reference in this datasheet.

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