Anti-METTL16 antibody (ab186012)
Key features and details
- Rabbit polyclonal to METTL16
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-METTL16 antibody
See all METTL16 primary antibodies -
Description
Rabbit polyclonal to METTL16 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide within Human METTL16 aa 350-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_076991.3
Database link: Q86W50 -
Positive control
- HeLa, 293T, Jurkat, TCMK-1, NIH3T3 wole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 64 kDa. |
Target
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Relevance
METT10D belongs to the methyltransferase superfamily, METT10D/rlmF family. The exact function of METT10D remains unknown. There are two named isoforms. -
Database links
- Entrez Gene: 101098176 Cat
- Entrez Gene: 468142 Chimpanzee
- Entrez Gene: 100765063 Chinese hamster
- Entrez Gene: 480655 Dog
- Entrez Gene: 101136835 Gorilla
- Entrez Gene: 100734997 Guinea pig
- Entrez Gene: 79066 Human
- Entrez Gene: 67493 Mouse
see all -
Alternative names
- 2610100D03Rik antibody
- 2810013M15Rik antibody
- A830095F14Rik antibody
see all
Images
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All lanes : Anti-METTL16 antibody (ab186012) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 3 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab186012 has been referenced in 4 publications.
- Chen PB et al. Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through m6A Modification of MAT2A Pre-mRNA by METTL16. Oxid Med Cell Longev 2022:4036274 (2022). PubMed: 35069973
- Mendel M et al. Splice site m6A methylation prevents binding of U2AF35 to inhibit RNA splicing. Cell 184:3125-3142.e25 (2021). PubMed: 33930289
- Jabs S et al. Impact of the gut microbiota on the m6A epitranscriptome of mouse cecum and liver. Nat Commun 11:1344 (2020). PubMed: 32165618
- Mendel M et al. Methylation of Structured RNA by the m6A Writer METTL16 Is Essential for Mouse Embryonic Development. Mol Cell 71:986-1000.e11 (2018). PubMed: 30197299