Anti-METTL21D antibody (ab121127)

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Overview

  • Product name

    Anti-METTL21D antibody

  • Description

    Rabbit polyclonal to METTL21D

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment, corresponding to amino acids 3-143 of Human METTL21D (Q9H867-1).

  • Positive control

    • Human breast tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (retrieval buffer pH 6).

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-METTL21D antibody (ab121127)
    Immunocytochemistry/ Immunofluorescence - Anti-METTL21D antibody (ab121127)
    Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121127 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL21D antibody (ab121127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL21D antibody (ab121127)
    ab121127 at 1/125 dilution staining METTL21D in Paraffin-emebedded Human breast tissue by Immunohistochemistry.

References

ab121127 has not yet been referenced specifically in any publications.

Publishing research using ab121127? Please let us know so that we can cite the reference in this datasheet.

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