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    mettl21d-antibody-ab121127.pdf

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Anti-METTL21D antibody (ab121127)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-METTL21D antibody (ab121127)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL21D antibody (ab121127)

Key features and details

  • Rabbit polyclonal to METTL21D
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Protein
Product image
Recombinant Human METTL21D protein (ab187477)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-METTL21D antibody
  • Description

    Rabbit polyclonal to METTL21D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 3-143 of Human METTL21D (Q9H867-1).

  • Positive control

    • Human breast tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human METTL21D protein (ab187477)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab121127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (retrieval buffer pH 6).
Notes
ICC/IF
Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (retrieval buffer pH 6).

Target

  • Function

    Probable methyltransferase.
  • Sequence similarities

    Belongs to the methyltransferase superfamily. METTL21 family.
  • Target information above from: UniProt accession Q9H867 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 79609 Human
    • Entrez Gene: 207965 Mouse
    • Entrez Gene: 362748 Rat
    • Omim: 615260 Human
    • SwissProt: Q9H867 Human
    • SwissProt: Q8C436 Mouse
    • Unigene: 558541 Human
    • Alternative names

      • C14orf138 antibody
      • Methyltransferase like 21D antibody
      • Methyltransferase-like protein 21D antibody
      • Mettl21d antibody
      • MT21D_HUMAN antibody
      • Protein lysine methyltransferase METTL21D antibody
      • Valosin containing protein lysine (K) methyltransferase antibody
      • Valosin containing protein lysine methyltransferase antibody
      • VCP KMT antibody
      • VCP lysine methyltransferase antibody
      • VCPKMT antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-METTL21D antibody (ab121127)
      Immunocytochemistry/ Immunofluorescence - Anti-METTL21D antibody (ab121127)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121127 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL21D antibody (ab121127)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL21D antibody (ab121127)
      ab121127 at 1/125 dilution staining METTL21D in Paraffin-emebedded Human breast tissue by Immunohistochemistry.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab121127? Please let us know so that we can cite the reference in this datasheet.

    ab121127 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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