Overview

  • Product name
    Anti-METTL21D antibody
  • Description
    Rabbit polyclonal to METTL21D
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 3-143 of Human METTL21D (Q9H867-1).

  • Positive control
    • Human breast tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (retrieval buffer pH 6).

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-METTL21D antibody (ab121127)
    Immunocytochemistry/ Immunofluorescence - Anti-METTL21D antibody (ab121127)
    Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121127 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL21D antibody (ab121127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL21D antibody (ab121127)
    ab121127 at 1/125 dilution staining METTL21D in Paraffin-emebedded Human breast tissue by Immunohistochemistry.

References

ab121127 has not yet been referenced specifically in any publications.

Publishing research using ab121127? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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