Key features and details
- Rabbit polyclonal to METTL8
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-METTL8 antibody
See all METTL8 primary antibodies
DescriptionRabbit polyclonal to METTL8
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- IHC: Human stomach tissue. ICC/IF: Human cell line A-431 WB: Human cell line HepG2
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 33 kDa.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceMETTL8 displays signature motifs characteristic of nuclear receptor coregulators and chromatin remodeling enzymes
Cellular localizationCytoplasmic and Nuclear
- tension induced inhibited protein antibody
- methyltransferase like 8 antibody
- methyltransferase like protein 8 antibody
- TIP antibody
Anti-METTL8 antibody (ab122273) at 0.4 µg/ml + Human cell line HepG2
Predicted band size: 33 kDa
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab122273 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab1222273, at 1/15 dilution, staining METTL8 in Paraffin-embedded Human stomach tissue by Immunohistochemistry.
ab122273 has not yet been referenced specifically in any publications.