Overview

  • Product name

  • Description

    Rabbit polyclonal to MFAP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide corresponding to C terminal amino acids 225-255 of Human MFAP4, conjugated to KLH.

  • Positive control

    • Human lung carcinoma tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 29 kDa.
IHC-P 1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

Target

Images

  • Anti-MFAP4 antibody (ab103925) at 1/1000 dilution + human lung tissue lysate at 35 µg

    Secondary
    goat anti-rabbit IgG H&L (HRP) at 1/5000 dilution

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling MFAP4 with ab103925 at a 1/25 dilution. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Flow cytometric analysis of MCF-7 cells labeling MFAP4 with ab103925 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:

  • Moon EH  et al. Essential role for TMEM100 in vascular integrity but limited contributions to the pathogenesis of hereditary haemorrhagic telangiectasia. Cardiovasc Res 105:353-60 (2015). Read more (PubMed: 25538155) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver, Muscle, Tendon)
Permeabilization
Yes - Tween 20
Specification
Liver, Muscle, Tendon
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde

Daniel Tausan

Verified customer

Submitted Jun 14 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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