Overview

  • Product name

  • Description

    Rabbit polyclonal to MFF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Orangutan
  • Immunogen

    Synthetic peptide within Human MFF aa 100-150. The exact sequence is proprietary.
    Database link: Q9GZY8

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat cell lysate. IP: HEK-293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 38 kDa.
IP Use at an assay dependent concentration.

20 µl/mg lysate

Target

Images

  • MFF was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241597 at 20 µl/reaction. Western blot was performed from the immunoprecipitate using ab241597 at 1/500 dilution.

    Lane 1: ab241597 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

    Lysates prepared in NETN buffer.

  • All lanes : Anti-MFF antibody (ab241597) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 38 kDa


    Exposure time: 30 seconds


    Lysates prepared in NETN buffer.

References

ab241597 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab241597.
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