Product nameAnti-MFF antibody
See all MFF primary antibodies
DescriptionRabbit polyclonal to MFF
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human, Zebrafish
Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
- Fetal muscle lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab81127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionPlays a role in mitochondrial and peroxisomal fission.
Tissue specificityHighly expressed in heart, kidney, liver, brain, muscle, and stomach.
Sequence similaritiesBelongs to the tango11 family.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- C2orf33 antibody
- Chromosome 2 open reading frame 33 antibody
- DKFZp666J168 antibody
Anti-MFF antibody (ab81127) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal muscle lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Gel concentration 12%
All lanes : Anti-MFF antibody (ab81127) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Lane 4 : Zebrafish liver homogenate at 20 µg
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ab81127 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab81127 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Gilmore-Hall S et al. CCP1 promotes mitochondrial fusion and motility to prevent Purkinje cell neuron loss in pcd mice. J Cell Biol 218:206-219 (2019). Read more (PubMed: 30337352) »
- Moore TM et al. The impact of exercise on mitochondrial dynamics and the role of Drp1 in exercise performance and training adaptations in skeletal muscle. Mol Metab N/A:N/A (2018). Read more (PubMed: 30591411) »