Overview

  • Product name

  • Description

    Rabbit polyclonal to MFF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human, Zebrafish
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 287-336 (LTVVDAASLR RQIIKLNRRL QLLEEENKER AKREMVMYSI TVAFWLLNSW) of Human MFF (NP_064579).

  • Positive control

    • Fetal muscle lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549

Properties

Applications

Our Abpromise guarantee covers the use of ab81127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-MFF antibody (ab81127) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Gel concentration 12%
  • All lanes : Anti-MFF antibody (ab81127) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 20 µg
    Lane 4 : Zebrafish liver homogenate at 20 µg

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes
  • ab81127 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab81127 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Gilmore-Hall S  et al. CCP1 promotes mitochondrial fusion and motility to prevent Purkinje cell neuron loss in pcd mice. J Cell Biol 218:206-219 (2019). Read more (PubMed: 30337352) »
  • Moore TM  et al. The impact of exercise on mitochondrial dynamics and the role of Drp1 in exercise performance and training adaptations in skeletal muscle. Mol Metab N/A:N/A (2018). Read more (PubMed: 30591411) »
See all 10 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (mouse heart fractions)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
70 µg
Specification
mouse heart fractions
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 30 2018

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (p38 mouse melanoma cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Treatment
Mff KD siRNA
Specification
p38 mouse melanoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Ms. Carmen Adriaens

Verified customer

Submitted Aug 07 2015

Answer


The immunogens differ, though both are closer to the C terminus than the N terminus. The immunogen for ab81127 is derived from within (but is not identical to) the range given on the datasheet, amino acids 287-336 of human MFF. The immunogen for ab129075 is a peptide derived from the range of amino acids 270 -300 of human MFF (RYGISNIDTTIEGTSDDLTVVDAASLRRQI).

Read More
Application
Western blot
Sample
Rat Cell lysate - whole cell (Neurones primaires corticaux)
Loading amount
1.2e+006 cells
Specification
Neurones primaires corticaux
Gel Running Conditions
Reduced Denaturing (gel 15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mrs. Marlene Daloyau

Verified customer

Submitted Oct 16 2012

Answer

Code de remise pour le test de ab74257: *******
Code de remise pour le test de ab81127 : ****** (ci-joint l'alignement de la région aa 30-100 avec la protéine de rat)
Code de remise pour le test de ab101908 : *******

Date d’expiration pour ces 2 codes : *****

Je suis ravi que vous souhaitiez participerà notre offre et tester ab74257, ab81127 et ab101908chez le rat. Les codes de réductionsont utilisables sous 120 jours et pour l’achat de 3 autres anticorps primaires.

Vous devez dans un premier temps soumettre une Abreview avec vos résultatschez le rat pour chaque anticorpsen notant le code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de l'anticorps testé.

Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches.

Termes et Conditions : https://www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us.

Regarding antibodies ab79397 and ab32577, they both have been tested in IHC with paraffin embedded sections in the species stated on the datasheet. We don’t have preliminary data using them with frozen sections, but they are very likely to react in such conditions.

Ab48139 has recently been validated to use in IHC-P in Canine, Feline, Mouse, Rat and Human.

As for ab81127, ab83015 and ab98975, unfortunately, we have not characterised them by IHC, it doesn’t mean they won’t work in this application, but we don’t have data to support it.

At Abcam we have an antibody evaluation system, called Abreview, by which customers interested in testing an antibody in new species or applications (not yet tested) can benefit from a free primary antibody in their next purchase if they share the results with us, publishing them in our website.

To participate in this system, customers should first let us know the antibody they want to test in the new species/ applications. We then issue the discount code valid for one free primary antibody. This code must be issued before purchasing the antibody. The customer then purchases the antibody normally, and tests it in the new species / applications.

Once we receive the results in our Website as an Abreview, the discount code becomes active, and it can be redeemed for any primary antibody ordered within the next 4 months.

As mentioned, the discount code might be issued before the purchase of the antibody, but, as an exceptional case, I can still send a discount code if you would like to test any of these antibodies (ab81127, ab83015 and ab98975) with IHC and share your results with us.

To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: http://it.adminsite.com/admin/crm2/www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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