Recombinant Anti-MFF antibody [EPR7360] (ab129075)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7360] to MFF
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MFF antibody [EPR7360]
See all MFF primary antibodies -
Description
Rabbit monoclonal [EPR7360] to MFF -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human MFF aa 250-350. The exact sequence is proprietary.
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Positive control
- Fetal muscle and fetal heart lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7360 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa). |
Target
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Function
Plays a role in mitochondrial and peroxisomal fission. -
Tissue specificity
Highly expressed in heart, kidney, liver, brain, muscle, and stomach. -
Sequence similarities
Belongs to the tango11 family. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 56947 Human
- Entrez Gene: 75734 Mouse
- Entrez Gene: 301563 Rat
- Omim: 614785 Human
- SwissProt: Q9GZY8 Human
- SwissProt: Q6PCP5 Mouse
- SwissProt: Q4KM98 Rat
- Unigene: 471528 Human
see all -
Alternative names
- C2orf33 antibody
- Chromosome 2 open reading frame 33 antibody
- DKFZp666J168 antibody
see all
Images
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All lanes : Anti-MFF antibody [EPR7360] (ab129075) at 1/1000 dilution
Lane 1 : Fetal muscle lysates
Lane 2 : Fetal heart lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab129075 has been referenced in 10 publications.
- Basei FL et al. Nek4 regulates mitochondrial respiration and morphology. FEBS J 289:3262-3279 (2022). PubMed: 34986513
- Wang Y et al. Low-Dose Delta-9-Tetrahydrocannabinol as Beneficial Treatment for Aged APP/PS1 Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35269905
- Simula L et al. PD-1-induced T cell exhaustion is controlled by a Drp1-dependent mechanism. Mol Oncol 16:188-205 (2022). PubMed: 34535949
- Prola A et al. Isolation and Phospholipid Enrichment of Muscle Mitochondria and Mitoplasts. Bio Protoc 11:e4201 (2021). PubMed: 34761073
- Gordillo GM et al. Mitochondria as Target for Tumor Management of Hemangioendothelioma. Antioxid Redox Signal 34:137-153 (2021). PubMed: 32597200
- Martínez J et al. Mitofusins modulate the increase in mitochondrial length, bioenergetics and secretory phenotype in therapy-induced senescent melanoma cells. Biochem J 476:2463-2486 (2019). PubMed: 31431479
- Li B et al. Interval and continuous exercise overcome memory deficits related to ß-Amyloid accumulation through modulating mitochondrial dynamics. Behav Brain Res 376:112171 (2019). PubMed: 31445975
- Simula L et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep 25:3059-3073.e10 (2018). PubMed: 30540939
- Song M et al. Abrogating Mitochondrial Dynamics in Mouse Hearts Accelerates Mitochondrial Senescence. Cell Metab 26:872-883.e5 (2017). PubMed: 29107503
- Suliman HB et al. Mitochondrial quality control in alveolar epithelial cells damaged by S. aureus pneumonia in mice. Am J Physiol Lung Cell Mol Physiol 313:L699-L709 (2017). PubMed: 28663335