Overview

  • Product name

    Anti-MFF antibody [EPR7360]
    See all MFF primary antibodies
  • Description

    Rabbit monoclonal [EPR7360] to MFF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MFF aa 250-350. The exact sequence is proprietary.

  • Positive control

    • Fetal muscle and fetal heart lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-MFF antibody [EPR7360] (ab129075) at 1/1000 dilution

      Lane 1 : Fetal muscle lysates
      Lane 2 : Fetal heart lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 38 kDa
      Observed band size: 34 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Simula L  et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep 25:3059-3073.e10 (2018). Read more (PubMed: 30540939) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Sample
    Rat Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Sep 09 2013

    Answer


    The immunogens differ, though both are closer to the C terminus than the N terminus. The immunogen for ab81127 is derived from within (but is not identical to) the range given on the datasheet, amino acids 287-336 of human MFF. The immunogen for ab129075 is a peptide derived from the range of amino acids 270 -300 of human MFF (RYGISNIDTTIEGTSDDLTVVDAASLRRQI).

    Read More

    Question

    Dear abcam technical team,

    Here is the answer to your questionary. I hope you can solve my problem. Best regards.

    It will take approximately 5 minutes to complete the questionnaire. Please fill-in all fields so that we can better assist you.



    1) Abcam product code ab: 5766-1



    2) Abcam order reference number or product batch number: #YI110107S



    3) Description of the problem: We tested the antibody by WB with lysates from different cell types coming from mouse and human. None of them were positive for the antibody. We try different dilutions as well and nothing.



    4) Sample preparation:

    Type of sample (whole cell lysates, fraction, recombinant protein…): whole cell lysates

    Lysis buffer: RIPA

    Protease inhibitors: PIC

    Phosphatase inhibitors: no

    Reducing agent: NuPAGE LDS sample buffer (invitrogen NP0008) + 2-mercaptoethanol

    Boiling for ≥5 min? yes

    Protein loaded ug/lane or cells/lane: 50 ug/lane

    Positive control: use of different cell lines: astrocytes, human t cells and MEFs, all of them with endogenous expression of MFF.

    Negative control: no



    5) Percentage of gel: 4-12% in MOPS buffer

    Type of membrane: Nitrocellulose

    Protein transfer verified: yes, with Ponceau red. Also the same membranes, after blotting against MFF, were positive for other antibodies.

    Blocking agent and concentration: milk 5%

    Blocking time 30 min-1 hour

    Blocking temperature: 25°C



    6) Primary antibody (If more than one was used, describe in “additional notes”):

    Concentration or dilution 1:100 to 1:1000

    Diluent buffer: TBS-tween 0.1% + 5% milk

    Incubation time: over night

    Incubation temperature: 4°C



    7) Secondary antibody:

    Species: goat

    Reacts against: rabbit and mouse

    Concentration or dilution: 1:4000

    Diluent buffer : : TBS-tween 0.1% + 5% milk

    Incubation time: 1 hour

    Incubation temperature: 25°C

    Fluorochrome or enzyme conjugate: HRP



    8) Washing after primary and secondary antibodies:

    Buffer : TBS-tween 0.1%

    Number of washes: 3 times of 15 min each



    9) Detection method: ECL



    10) How many times have you run this staining? 3

    Do you obtain the same results every time? yes

    What steps have you altered to try and optimize the use of this antibody? Concentration of antibodies (primary and secondary), sensitivity of ECL.

    Read More
    Answer

    Thank you for your enquiry regarding ab129075 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    The reference number you have provided (YI110107S) is invalid and our system does not recognize it.

    Could you please confirm if you purchased this product directly from Abcam or via a distributor?
    Furthermore, could you provide the date of arrival?
    Does the detection system work fine? Have you used it successfully with another primary antibody?



    I look forward to hearing from you soon.

    Read More

    Answer

    I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

    In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

    As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

    All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

    Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

    Read More

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