Overview

  • Product name

  • Description

    Rabbit polyclonal to MFSD2A/NLS1
  • Host species

    Rabbit
  • Specificity

    ab105399 is predicted not to cross-react with other MFSD family members.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MFSD2A/NLS1 (C terminal).
    (Peptide available as ab199848)

  • Positive control

    • Rat lung tissue lysate.
  • General notes

     This product was previously labelled as MFSD2A

     

Properties

Applications

Our Abpromise guarantee covers the use of ab105399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
ELISA Use at an assay dependent concentration.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse lung tissue staining MFSD2A/NLS1 with ab105399 at 20µg/ml

  • Lane 1 : Anti-MFSD2A/NLS1 antibody (ab105399) at 1 µg/ml
    Lane 2 : Anti-MFSD2A/NLS1 antibody (ab105399) at 2 µg/ml

    All lanes : Rat lung tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 60 kDa

  • Immunohistochemistry of MFSD2A/NLS1 in rat lung tissue with ab105399 antibody at 5 µg/ml.

  • Immunofluorescence of MFSD2A/NLS1 in Rat Lung cells using ab105399 at 20 ug/ml.

References

This product has been referenced in:

  • López-Vicario C  et al. Leukocytes from obese individuals exhibit an impaired SPM signature. FASEB J 33:7072-7083 (2019). Read more (PubMed: 30840838) »
  • Awad H  et al. MiR-155 deletion reduces ischemia-induced paralysis in an aortic aneurysm repair mouse model: Utility of immunohistochemistry and histopathology in understanding etiology of spinal cord paralysis. Ann Diagn Pathol 36:12-20 (2018). Read more (PubMed: 29966831) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 5 minute(s) · Concentration: 1%
Fixative
4% Paraformaldehyde and 0.1% glutaraldehyde

Abcam user community

Verified customer

Submitted Jun 03 2016

Answer

I am very pleased to hear you would like to test ab105399 and ab117618 in IHC and mouse, respectively. This code will give you $337 off an order and $385 off another order before the expiration date. To redeem this offer, please submit an Abreview for IHC and for mouse and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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