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    mg-132-proteasome-inhibitor-ab141003.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome
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MG-132, proteasome inhibitor (ab141003)

  • Datasheet
  • SDS
  • COA
Reviews (1) Submit a question References (15)

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Western blot - MG-132, proteasome inhibitor (ab141003)
  • Immunocytochemistry/ Immunofluorescence - MG-132, proteasome inhibitor (ab141003)
  • Flow Cytometry - MG-132, proteasome inhibitor (ab141003)
  • Immunoprecipitation - MG-132, proteasome inhibitor (ab141003)
  • Western blot - MG-132, proteasome inhibitor (ab141003)
  • Western blot - MG-132, proteasome inhibitor (ab141003)
  • Western blot - MG-132, proteasome inhibitor (ab141003)
  • Western blot - MG-132, proteasome inhibitor (ab141003)
  • Western blot - MG-132, proteasome inhibitor (ab141003)

Key features and details

  • Potent, reversible proteasome inhibitor
  • CAS Number: 133407-82-6
  • Purity: > 98%
  • Soluble in DMSO to 100 mM but unstable for prolonged periods. Soluble in ethanol to 100 mM.

  • Form / State: Solid
  • Source: Synthetic

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Overview

  • Product name

    MG-132, proteasome inhibitor
  • Description

    Potent, reversible proteasome inhibitor
  • Alternative names

    • Z-LLL-CHO
  • Biological description

    Potent, reversible, proteasome inhibitor (Ki = 4 nM). Inhibits NF-κB activation by preventing IκB degradation (IC50 = 3 μM). Anti-cancer properties in vitro and in vivo. Cell-permeable.


    Also available as ethanol solution (ab147047).

  • Purity

    > 98%
  • CAS Number

    133407-82-6
  • Chemical structure

    Chemical Structure

Properties

  • Molecular weight

    475.62
  • Molecular formula

    C26H41N3O5
  • Sequence

    LLL (Modifications: N-terminal benzyloxycarbonyl; C-terminal aldehyde)
  • PubChem identifier

    462382
  • Storage instructions

    Store at -20°C. It is important to note that this is air sensitive and impurities can occur as a result of air oxidation. Store under desiccating conditions.
  • Solubility overview

    Soluble in DMSO to 100 mM but unstable for prolonged periods. Soluble in ethanol to 100 mM.

  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC(C)CC(C=O)NC(=O)C(CC(C)C)NC(=O)C(CC(C)C)NC(=O)OCC1=CC=CC=C1
  • Source

    Synthetic

  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Pharmacology
    • Enzymes
    • Protease
    • Proteasome
    • Inhibitors

Associated products

    Images

    • Western blot - MG-132, proteasome inhibitor (ab141003)
      Western blot - MG-132, proteasome inhibitor (ab141003)
      All lanes : Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (ab211324) at 1/1000 dilution

      Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HeLa whole cell lysate treated with 2µM MG-132 (ab141003) for 18 hours
      Lane 3 : HeLa whole cell lysate treated with 2µM MG-132 (ab141003) for 18 hours, then treated with Alkaline Phosphatase for 1 hour

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 62 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - MG-132, proteasome inhibitor (ab141003)
      Immunocytochemistry/ Immunofluorescence - MG-132, proteasome inhibitor (ab141003)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, treated with 2μM MG-132 (ab141003) for 18 hours or untreated, labeling SQSTM1 / p62 (phospho S349) with ab211324 at 1/100 dilution, followed by Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) secondary antibody at 1/200 dilution (green).

      Confocal image showing cytoplasmic staining on HeLa cell line. The expression increased after treatment with 2μM MG-132 (ab141003) for 18 hours.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Flow Cytometry - MG-132, proteasome inhibitor (ab141003)
      Flow Cytometry - MG-132, proteasome inhibitor (ab141003)

      Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, treated with 2μM MG-132 (ab141003) for 18 hours (red) or untreated (green), labeling SQSTM1 / p62 (phospho S349) with ab211324 at 1/500 dilution compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    • Immunoprecipitation - MG-132, proteasome inhibitor (ab141003)
      Immunoprecipitation - MG-132, proteasome inhibitor (ab141003)

      SQSTM1 / p62 (phospho S349) was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 2μM MG-132 (ab141003) for 18h with ab211324 at 1/30 dilution.

      Western blot was performed from the immunoprecipitate using ab211324 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: HeLa treated with 2μM MG-132 (ab141003) for 18h whole cell lysate, 10 µg (Input).
      Lane 2: ab211324 IP in HeLa treated with 2μM MG-132 (ab141003) for 18h whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211324 in HeLa treated with 2μM MG-132 (ab141003) for 18h whole cell lysate.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - MG-132, proteasome inhibitor (ab141003)
      Western blot - MG-132, proteasome inhibitor (ab141003)
      All lanes : Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (ab211324) at 1/1000 dilution

      Lane 1 : Untreated C6 (Rat glial tumor cell line) whole cell lysate
      Lane 2 : C6 whole cell lysate treated with 2µM MG-132 (ab141003) for 18 hours

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 62 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - MG-132, proteasome inhibitor (ab141003)
      Western blot - MG-132, proteasome inhibitor (ab141003)
      All lanes : Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (ab211324) at 1/1000 dilution

      Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
      Lane 2 : NIH/3T3 whole cell lysate treated with 2µM MG-132 (ab141003) for 18 hours

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 62 kDa why is the actual band size different from the predicted?


      Exposure time: 4 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - MG-132, proteasome inhibitor (ab141003)
      Western blot - MG-132, proteasome inhibitor (ab141003)
      All lanes : Anti-HIF-1 alpha antibody [EPR16897] (ab179483) at 0.163 µg/ml

      Lane 1 : Untreated C6 (rat glial tumor glial cell), whole cell lysate
      Lane 2 : C6 treated with 400 µM CoCl2 and 200 µM MG-132 (ab141003) for 4 hours

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 110 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      Blocking and diluting buffer: 5% NFDM/TBST.

      The expression of HIF-1 alpha is induced by CoCl2 and maintained by MG-132 (PMID: 15836611).

    • Western blot - MG-132, proteasome inhibitor (ab141003)
      Western blot - MG-132, proteasome inhibitor (ab141003)
      All lanes : Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/5000 dilution

      Lane 1 : Untreated HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg
      Lane 2 : HT-1080 treated with 10µM MG-132 (ab141003) for 4 hours, whole cell lysate at 10 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Exposure time: 103 seconds


      Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    • Western blot - MG-132, proteasome inhibitor (ab141003)
      Western blot - MG-132, proteasome inhibitor (ab141003)
      All lanes : Anti-ATF-4 antibody [EPR18111] (ab184909) at 1/1000 dilution

      Lane 1 : Untreated HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate (control)
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) treated with 5 µM MG-132 (ab141003) and 3 µg/ml tunicamycin (ab120296) for 6 hours whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 22095285).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download
    • COA

    References (15)

    Publishing research using ab141003? Please let us know so that we can cite the reference in this datasheet.

    ab141003 has been referenced in 15 publications.

    • Luciani A  et al. Impaired mitophagy links mitochondrial disease to epithelial stress in methylmalonyl-CoA mutase deficiency. Nat Commun 11:970 (2020). PubMed: 32080200
    • Baarsma HA  et al. Disruption of AKAP-PKA Interaction Induces Hypercontractility With Concomitant Increase in Proliferation Markers in Human Airway Smooth Muscle. Front Cell Dev Biol 8:165 (2020). PubMed: 32328490
    • Gain C  et al. Proteasomal inhibition triggers viral oncoprotein degradation via autophagy-lysosomal pathway. PLoS Pathog 16:e1008105 (2020). PubMed: 32092124
    • Chen L  et al. Ivermectin suppresses tumour growth and metastasis through degradation of PAK1 in oesophageal squamous cell carcinoma. J Cell Mol Med 24:5387-5401 (2020). PubMed: 32237037
    • Simpson LM  et al. Inducible Degradation of Target Proteins through a Tractable Affinity-Directed Protein Missile System. Cell Chem Biol 27:1164-1180.e5 (2020). PubMed: 32668203
    View all Publications for this product

    Customer reviews and Q&As

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    Good proteasome inhibitor

    Excellent
    Abreviews
    Abreviews
    HCT116 cells were incubated with the proteasome inhibitor MG132 or vehicle for 6 h and the total protein fractions were analyzed by Western blot. We observed an increase of specific proteins and also proteins binded to ubiquitin.

    Abcam user community

    Verified customer

    Submitted Oct 03 2018

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