Anti-MG53 antibody (ab154238)

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Overview

  • Product name

    Anti-MG53 antibody
    See all MG53 primary antibodies

  • Description

    Rabbit polyclonal to MG53

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment corresponding to Human MG53 aa 82-258.
    Database link: Q6ZMU5

  • Positive control

    • HCT116 whole cell lysate.

  • General notes

     This product was previously labelled as TRIM72

     

Properties

Applications

Our Abpromise guarantee covers the use of ab154238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.

Target

  • Function

    Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.

  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.

  • Post-translational
    modifications

    Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.

  • Cellular localization

    Cell membrane > sarcolemma. Cytoplasmic vesicle membrane. Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • LOC493829 antibody
    • Mg53 antibody
    • Mitsugumin-53 antibody
    • TRI72_HUMAN antibody
    • trim72 antibody
    • Tripartite motif containing 72 antibody
    • Tripartite motif containing protein 72 antibody
    • Tripartite motif-containing protein 72 antibody
    see all

Images

  • Western blot - Anti-MG53 antibody (ab154238)
    Western blot - Anti-MG53 antibody (ab154238)
    Anti-MG53 antibody (ab154238) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 53 kDa



    12% SDS PAGE

References

Publishing research using ab154238? Please let us know so that we can cite the reference in this datasheet.

ab154238 has not yet been referenced specifically in any publications.

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