Anti-MG53 antibody (ab154238)

Submit a review Submit a question
Western blot - Anti-MG53 antibody (ab154238)

    Key features and details

    • Rabbit polyclonal to MG53
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-MG53 antibody
      See all MG53 primary antibodies

    • Description

      Rabbit polyclonal to MG53

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat

    • Immunogen

      Recombinant fragment corresponding to Human MG53 aa 82-258.
      Database link: Q6ZMU5

    • Positive control

      • HCT116 whole cell lysate.

    • General notes

       This product was previously labelled as TRIM72

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab154238 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 53 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 53 kDa.

    Target

    • Function

      Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.

    • Sequence similarities

      Belongs to the TRIM/RBCC family.
      Contains 1 B box-type zinc finger.
      Contains 1 B30.2/SPRY domain.
      Contains 1 RING-type zinc finger.

    • Post-translational
      modifications

      Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.

    • Cellular localization

      Cell membrane > sarcolemma. Cytoplasmic vesicle membrane. Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • LOC493829 antibody
      • Mg53 antibody
      • Mitsugumin-53 antibody
      • TRI72_HUMAN antibody
      • trim72 antibody
      • Tripartite motif containing 72 antibody
      • Tripartite motif containing protein 72 antibody
      • Tripartite motif-containing protein 72 antibody
      see all

    Images

    • Western blot - Anti-MG53 antibody (ab154238)
      Western blot - Anti-MG53 antibody (ab154238)
      Anti-MG53 antibody (ab154238) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

      Predicted band size: 53 kDa



      12% SDS PAGE

    References (0)

    Publishing research using ab154238? Please let us know so that we can cite the reference in this datasheet.

    ab154238 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab154238.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com