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    mg53-antibody-ab154238.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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Anti-MG53 antibody (ab154238)

  • Datasheet
  • SDS
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Western blot - Anti-MG53 antibody (ab154238)

    Key features and details

    • Rabbit polyclonal to MG53
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-MG53 antibody
      See all MG53 primary antibodies
    • Description

      Rabbit polyclonal to MG53
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat
    • Immunogen

      Recombinant fragment corresponding to Human MG53 aa 82-258.
      Database link: Q6ZMU5

    • Positive control

      • HCT116 whole cell lysate.
    • General notes

       This product was previously labelled as TRIM72

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Regulation
      • Cell Biology
      • Other Antibodies
      • Other Antibodies

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human MG53 protein (ab132761)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab154238 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 53 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 53 kDa.

    Target

    • Function

      Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
    • Sequence similarities

      Belongs to the TRIM/RBCC family.
      Contains 1 B box-type zinc finger.
      Contains 1 B30.2/SPRY domain.
      Contains 1 RING-type zinc finger.
    • Post-translational
      modifications

      Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.
    • Cellular localization

      Cell membrane > sarcolemma. Cytoplasmic vesicle membrane. Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine.
    • Target information above from: UniProt accession Q6ZMU5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 493829 Human
      • Entrez Gene: 434246 Mouse
      • Entrez Gene: 365377 Rat
      • SwissProt: Q6ZMU5 Human
      • SwissProt: Q1XH17 Mouse
      • SwissProt: A0JPQ4 Rat
      • Unigene: 661859 Human
      • Unigene: 389924 Mouse
      • Unigene: 23565 Rat
      see all
    • Alternative names

      • LOC493829 antibody
      • Mg53 antibody
      • Mitsugumin-53 antibody
      • TRI72_HUMAN antibody
      • trim72 antibody
      • Tripartite motif containing 72 antibody
      • Tripartite motif containing protein 72 antibody
      • Tripartite motif-containing protein 72 antibody
      see all

    Images

    • Western blot - Anti-MG53 antibody (ab154238)
      Western blot - Anti-MG53 antibody (ab154238)
      Anti-MG53 antibody (ab154238) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

      Predicted band size: 53 kDa



      12% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab154238? Please let us know so that we can cite the reference in this datasheet.

    ab154238 has not yet been referenced specifically in any publications.

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