Anti-MG53 antibody (ab154238)
Key features and details
- Rabbit polyclonal to MG53
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MG53 antibody
See all MG53 primary antibodies -
Description
Rabbit polyclonal to MG53 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human MG53 aa 82-258.
Database link: Q6ZMU5 -
Positive control
- HCT116 whole cell lysate.
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General notes
This product was previously labelled as TRIM72
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
Target
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Function
Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles. -
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
Post-translational
modificationsDisulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization. -
Cellular localization
Cell membrane > sarcolemma. Cytoplasmic vesicle membrane. Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine. - Information by UniProt
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Database links
- Entrez Gene: 493829 Human
- Entrez Gene: 434246 Mouse
- Entrez Gene: 365377 Rat
- SwissProt: Q6ZMU5 Human
- SwissProt: Q1XH17 Mouse
- SwissProt: A0JPQ4 Rat
- Unigene: 661859 Human
- Unigene: 389924 Mouse
see all -
Alternative names
- LOC493829 antibody
- Mg53 antibody
- Mitsugumin-53 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154238 has not yet been referenced specifically in any publications.