Overview

  • Product name

    Anti-MG53 antibody (Biotin)
    See all MG53 primary antibodies
  • Description

    Goat polyclonal to MG53 (Biotin)
  • Host species

    Goat
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide corresponding to Human MG53 aa 61-70 (internal sequence). NP_001008275.1.
    Sequence:

    C-RPQALSTNLQ


    Database link: Q6ZMU5

  • Positive control

    • WB: Mouse heart lysate.
  • General notes

     This product was previously labelled as TRIM72

     

Properties

Applications

Our Abpromise guarantee covers the use of ab238252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 µg/ml. Predicted molecular weight: 53 kDa.

Target

  • Function

    Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications

    Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.
  • Cellular localization

    Cell membrane > sarcolemma. Cytoplasmic vesicle membrane. Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine.
  • Information by UniProt
  • Database links

  • Alternative names

    • LOC493829 antibody
    • Mg53 antibody
    • Mitsugumin-53 antibody
    • TRI72_HUMAN antibody
    • trim72 antibody
    • Tripartite motif containing 72 antibody
    • Tripartite motif containing protein 72 antibody
    • Tripartite motif-containing protein 72 antibody
    see all

Images

  • Anti-MG53 antibody (Biotin) (ab238252) at 0.01 µg/ml + Mouse heart lysate in RIPA buffer at 35 µg

    Predicted band size: 53 kDa



    Incubated with primary antibody for 1 hour.

    Detected by chemiluminiscence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

References

ab238252 has not yet been referenced specifically in any publications.

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