Recombinant Anti-MGA antibody [EPR19854] (ab214814)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-MGA antibody [EPR19854]
See all MGA primary antibodies -
Description
Rabbit monoclonal [EPR19854] to MGA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Mouse MGA aa 250-350. The exact sequence is proprietary.
Database link: A2AWL7 -
Positive control
- WB: Wild-type mouse embryonic stem (ES) cell lysate.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19854 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab214814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 400 kDa (predicted molecular weight: 328 kDa). |
Target
-
Relevance
MGA contains 1 basic helix-loop-helix (bHLH) domain and is a possible transcription factor. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 29808 Mouse
- SwissProt: A2AWL7 Mouse
- SwissProt: Q3TS36 Mouse
- SwissProt: Q9QXJ5 Mouse
-
Alternative names
- MAD5 antibody
- MAX dimerization protein 5 antibody
- MAX gene associated antibody
see all
Images
-
![Western blot - Anti-MGA antibody [EPR19854] (ab214814)](https://www.abcam.com/ps/products/214/ab214814/Images/ab214814-288239-anti-mga-antibody-epr19854-western-blot.jpg)
Western blot - Anti-MGA antibody [EPR19854] (ab214814)All lanes : Anti-MGA antibody [EPR19854] (ab214814) at 1/1000 dilution
Lane 1 : Wild-type mouse embryonic stem (ES) cell lysate
Lane 2 : MGA knockout mouse embryonic stem (ES) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 328 kDa
Observed band size: 400 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The cell lysates were kindly provided by our collaborator Dr. Jinzhong Qin, Nanjing University MARC.
Datasheets and documents
References
ab214814 has not yet been referenced specifically in any publications.
