Overview

  • Product name

    Anti-MGA antibody [EPR19854] - BSA and Azide free
    See all MGA primary antibodies
  • Description

    Rabbit monoclonal [EPR19854] to MGA - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse MGA aa 100-350. The exact sequence is proprietary.
    Database link: A2AWL7

  • Positive control

    • WB: Wild-type mouse embryonic stem (ES) cell lysate.
  • General notes

    Ab226861 is the carrier-free version of ab214814. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226861 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab226861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 400 kDa (predicted molecular weight: 328 kDa).

Target

  • Relevance

    MGA contains 1 basic helix-loop-helix (bHLH) domain and is a possible transcription factor.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • MAD5 antibody
    • MAX dimerization protein 5 antibody
    • MAX gene associated antibody
    • MAX gene associated protein antibody
    • Max interacting protein antibody
    • MGAM antibody
    • MXD5 antibody
    see all

Images

  • All lanes : Anti-MGA antibody [EPR19854] (ab214814) at 1/1000 dilution

    Lane 1 : Wild-type mouse embryonic stem (ES) cell lysate
    Lane 2 : MGA knockout mouse embryonic stem (ES) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 328 kDa
    Observed band size: 400 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator Dr. Jinzhong Qin, Nanjing University MARC.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214814).

References

ab226861 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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