Product nameAnti-MGA antibody [EPR19854] - BSA and Azide free
See all MGA primary antibodies
DescriptionRabbit monoclonal [EPR19854] to MGA - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide within Mouse MGA aa 100-350. The exact sequence is proprietary.
Database link: A2AWL7
- WB: Wild-type mouse embryonic stem (ES) cell lysate.
Ab226861 is the carrier-free version of ab214814. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab226861 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab226861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 400 kDa (predicted molecular weight: 328 kDa).|
RelevanceMGA contains 1 basic helix-loop-helix (bHLH) domain and is a possible transcription factor.
- MAD5 antibody
- MAX dimerization protein 5 antibody
- MAX gene associated antibody
All lanes : Anti-MGA antibody [EPR19854] (ab214814) at 1/1000 dilution
Lane 1 : Wild-type mouse embryonic stem (ES) cell lysate
Lane 2 : MGA knockout mouse embryonic stem (ES) cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 328 kDa
Observed band size: 400 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
The cell lysates were kindly provided by our collaborator Dr. Jinzhong Qin, Nanjing University MARC.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214814).
ab226861 has not yet been referenced specifically in any publications.