Anti-MGAT1 antibody (ab109552)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-MGAT1 antibody
See all MGAT1 primary antibodies -
Description
Goat polyclonal to MGAT1 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit -
Immunogen
Synthetic peptide:
C-QVEKVRTNDR
, corresponding to internal sequence amino acids 369-378 of Human MGAT1 (NP_002397.2). -
Positive control
- MGAT1 transfected HEK293 lysate
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa). |
Target
-
Function
Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. -
Tissue specificity
Appears to be present in all tissues. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 13 family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
-
Database links
- Entrez Gene: 4245 Human
- Entrez Gene: 81519 Rat
- Omim: 160995 Human
- SwissProt: P26572 Human
- SwissProt: P27115 Rabbit
- SwissProt: Q09325 Rat
- Unigene: 519818 Human
- Unigene: 2712 Rat
-
Alternative names
- 3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
- Alpha-1 antibody
- Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
see all
Images
-
All lanes : Anti-MGAT1 antibody (ab109552) at 1 µg/ml
Lane 1 : HEK293 lysate in RIPA buffer, overexpressing Human MGAT1 with C terminal MYC tag
Lane 2 : Mock transfected HEK293 lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
Protocols
Datasheets and documents
References
ab109552 has not yet been referenced specifically in any publications.