Anti-MGAT1 antibody (ab109552)

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Overview

  • Product name

    Anti-MGAT1 antibody
    See all MGAT1 primary antibodies

  • Description

    Goat polyclonal to MGAT1

  • Host species

    Goat

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit

  • Immunogen

    Synthetic peptide:

    C-QVEKVRTNDR

    , corresponding to internal sequence amino acids 369-378 of Human MGAT1 (NP_002397.2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • MGAT1 transfected HEK293 lysate

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

  • Tissue specificity

    Appears to be present in all tissues.

  • Pathway

    Protein modification; protein glycosylation.

  • Sequence similarities

    Belongs to the glycosyltransferase 13 family.

  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
      • Alpha-1 antibody
      • Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
      • GGNT1 antibody
      • GLCNAC TI antibody
      • GlcNAc-T I antibody
      • GLCT1 antibody
      • GLYT1 antibody
      • GNT 1 antibody
      • GNT-I antibody
      • Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase antibody
      • MGAT antibody
      • MGAT1 antibody
      • MGAT1_HUMAN antibody
      • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I antibody
      see all

    Images

    • Western blot - Anti-MGAT1 antibody (ab109552)
      Western blot - Anti-MGAT1 antibody (ab109552)
      All lanes : Anti-MGAT1 antibody (ab109552) at 1 µg/ml

      Lane 1 : HEK293 lysate in RIPA buffer, overexpressing Human MGAT1 with C terminal MYC tag
      Lane 2 : Mock transfected HEK293 lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 50 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 hour

    References

    ab109552 has not yet been referenced specifically in any publications.

    Publishing research using ab109552? Please let us know so that we can cite the reference in this datasheet.

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