Overview

  • Product name
  • Description
    Rabbit polyclonal to MGAT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C-terminal last 50 aa of MGAT1 (Human) conjugated to KLH

  • Positive control
    • Human hepatocarcinoma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 50 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function
    Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
  • Tissue specificity
    Appears to be present in all tissues.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 13 family.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
    • Alpha-1 antibody
    • Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
    • GGNT1 antibody
    • GLCNAC TI antibody
    • GlcNAc-T I antibody
    • GLCT1 antibody
    • GLYT1 antibody
    • GNT 1 antibody
    • GNT-I antibody
    • Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase antibody
    • MGAT antibody
    • MGAT1 antibody
    • MGAT1_HUMAN antibody
    • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I antibody
    see all

Images

  • Western blot analysis of ab38857 in Y79 cell line lysate. Western blotting 1:100~500.
  • Ab38857 at 1/50 dilution staining human hepatocarcinoma for MGAT1 expression. A peroxidase-conjugated secondary antibody, followed by AEC staining was used.

References

ab38857 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question

Dear technical support team: This customer has already purchased ab38857 (Anti-MGAT1 antibody), and after he conducted WB with dog and human sample, the result shows multiple bands, therefore he wants to ask for you help to offer any suggestion to him, I also attached the image in this letter, and his experiment step as follow:   1. Order details: ?Batch number: GR58431-1 ?po: 974354 ?Abcam product code: ab38857 ?Antibody storage conditions (temperature/reconstitution etc) -20℃  2. Please describe the problem (high background, wrong band size, more bands, no band etc).  Multiple bands 3. On what material are you testing the antibody in WB? * Species: canine and human * What’s cell line or tissue: MDCK * Cell extract or nuclear extract: cell extract * Purified protein or Recombinant protein:  3. The lysate ?How much protein was loaded: 20μg ?What lysis buffer was used: DAPI ?What protease inhibitors were used: ?What loading buffer was used: DTT ?Phosphatase inhibitors: ?Did you heat the samples: temperature and time: 95℃ 5mins 4.  Electrophoresis/Gel conditions/ Transfer conditions ?Reducing or non reducing gel:reducing gel ?Reducing agent: DTT ?Gel percentage: 10% ?Transfer conditions: (Type of membrane, Protein transfer verified):​ PVDF  5. Blocking conditions ?Buffer: PBST ?Blocking agent: milk, BSA, serum, what percentage: milk 10% ?Incubation time: 1hr ?Incubation temperature: room temperature  6. Primary Antibody ?Species: rabbit ?Reacts against: canine At what dilution(s) have you tested this antibody: 200x and 500x What dilution buffer was used: PBS Incubation time: 1hr * Incubation temperature:room temperature * What washing steps were done: PBST 5 mins 3 times  7. Secondary Antibody ?Species: ?Reacts against: rabbit ?At what dilution(s) have you tested this antibody: 5000x ?Incubation time: 1hr ?Wash steps: PBST 5 mins 3 times ?Fluorochrome or enzyme conjugate: enzyme Do you know whether the problems you are experiencing come from the secondary? No   8. Detection method ECl, ECl+, other detection method: ECl+   9. Did you apply positive and negative controls along with the samples? Please specify. With positive control HuH7 cell lysate 20μg 10. Optimization attempts * How many times have you tried the Western? 3 times * Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control):   no * Do you obtain the same results every time e.g. are background bands always in the same place? Yes * What steps have you altered? Concentration of primary antibody   Could you please help this customer to solve the problem? Thanks for your kindly help Best regards  

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Answer

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. This antibody has only been tested with human samples only; so far we do not have any data regarding its cross-reactivity with other species such as dog (canine). Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem.   What lysis buffer was used: DAPI Question: Why DAPI was used? What is the composition of this buffer, please clarify with your customer.   What loading buffer was used: DTT Question: What is the composition of this buffer?   Blocking agent: milk 10% Question: Has your client tried other blocking agent like 5 % BSA in TBS-T rather than milk which for certain target proteins works better?   Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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