Recombinant Anti-MGAT1 antibody [EPR14247] (ab180578)
![Western blot - Anti-MGAT1 antibody [EPR14247] (ab180578)](https://www.abcam.com/ps/products/180/ab180578/Images/ab180578-215375-anti-mgat1-antibody-epr14247-western-blot.jpg "Western blot - Anti-MGAT1 antibody [EPR14247] (ab180578)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14247] to MGAT1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MGAT1 antibody [EPR14247]
See all MGAT1 primary antibodies -
Description
Rabbit monoclonal [EPR14247] to MGAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293 and HeLa whole cell lysate (ab150035).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14247 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
Target
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Function
Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. -
Tissue specificity
Appears to be present in all tissues. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 13 family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 4245 Human
- Omim: 160995 Human
- SwissProt: P26572 Human
- Unigene: 519818 Human
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Alternative names
- 3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
- Alpha-1 antibody
- Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
see all
Images
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Western blot - Anti-MGAT1 antibody [EPR14247] (ab180578)Anti-MGAT1 antibody [EPR14247] (ab180578) at 1/10000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 51 kDa -
![Western blot - Anti-MGAT1 antibody [EPR14247] (ab180578)](https://www.abcam.com/ps/products/180/ab180578/Images/ab180578-215374-anti-mgat1-antibody-epr14247-western-blot.jpg)
Western blot - Anti-MGAT1 antibody [EPR14247] (ab180578)Anti-MGAT1 antibody [EPR14247] (ab180578) at 1/2000 dilution + 293 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit igG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 51 kDa -
![Anti-MGAT1 antibody [EPR14247] (ab180578)](https://www.abcam.com/ps/products/180/ab180578/Images/ab180578-3-benefits-of-recombinant-antibodies.png)
Anti-MGAT1 antibody [EPR14247] (ab180578)
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (9)
ab180578 has been referenced in 9 publications.
- Song N et al. N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells. PLoS Pathog 17:e1009244 (2021). PubMed: 33539469
- Khakurel A et al. The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery. Mol Biol Cell 32:1594-1610 (2021). PubMed: 34161137
- Biswas B et al. Transgenic Rescue of Spermatogenesis in Males With Mgat1 Deleted in Germ Cells. Front Cell Dev Biol 8:212 (2020). PubMed: 32300591
- Zhang G et al. N-acetylglucosaminyltransferase-I as a novel regulator of epithelial-mesenchymal transition. FASEB J 33:2823-2835 (2019). PubMed: 30307765
- Fisher P et al. Modeling Glycan Processing Reveals Golgi-Enzyme Homeostasis upon Trafficking Defects and Cellular Differentiation. Cell Rep 27:1231-1243.e6 (2019). PubMed: 31018136
- D'Souza Z et al. Defects in COG-Mediated Golgi Trafficking Alter Endo-Lysosomal System in Human Cells. Front Cell Dev Biol 7:118 (2019). PubMed: 31334232
- Frisbie CP et al. Post-ER Stress Biogenesis of Golgi Is Governed by Giantin. Cells 8:N/A (2019). PubMed: 31847122
- Stewart SE et al. A genome-wide CRISPR screen reconciles the role of N-linked glycosylation in galectin-3 transport to the cell surface. J Cell Sci 130:3234-3247 (2017). PubMed: 28775154
- Casey CA et al. Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation. Alcohol Clin Exp Res 40:2573-2590 (2016). PubMed: 27748959
