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    mgat2-antibody-ab228950.pdf

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Signal Transduction Metabolism Lipid metabolism
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Anti-MGAT2 antibody (ab228950)

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  • SDS
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Western blot - Anti-MGAT2 antibody (ab228950)

    Key features and details

    • Rabbit polyclonal to MGAT2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-MGAT2 antibody
      See all MGAT2 primary antibodies
    • Description

      Rabbit polyclonal to MGAT2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment within Human MGAT2 (internal sequence). The exact sequence is proprietary.
      Database link: Q3SYC2

    • Positive control

      • WB: Raji whole cell lysate.
    • General notes

      Protein previously labeled as MOGAT2.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Lipid metabolism

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Raji whole cell lysate (ab30124)

    Applications

    Our Abpromise guarantee covers the use of ab228950 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.

    Target

    • Function

      Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity.
    • Tissue specificity

      Highly expressed in liver, small intestine, colon, stomach and kidney.
    • Pathway

      Glycerolipid metabolism; triacylglycerol biosynthesis.
    • Sequence similarities

      Belongs to the diacylglycerol acyltransferase family.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Target information above from: UniProt accession Q3SYC2 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 80168 Human
      • Omim: 610270 Human
      • SwissProt: Q3SYC2 Human
      • Unigene: 288568 Human
      • Alternative names

        • 2 acylglycerol O acyltransferase 2 antibody
        • 2-acylglycerol O-acyltransferase 2 antibody
        • Acyl CoA:monoacylglycerol acyltransferase 2 antibody
        • Acyl-CoA:monoacylglycerol acyltransferase 2 antibody
        • DC5 antibody
        • DGAT2L5 antibody
        • Diacylglycerol acyltransferase 2 like protein 5 antibody
        • Diacylglycerol acyltransferase 2-like protein 5 antibody
        • Diacylglycerol O acyltransferase candidate 5 antibody
        • Diacylglycerol O-acyltransferase candidate 5 antibody
        • EC 2.3.1.22 antibody
        • FLJ22644 antibody
        • hDC5 antibody
        • hMGAT2 antibody
        • Mgat1l antibody
        • MGAT2 antibody
        • MGC119183 antibody
        • MGC119184 antibody
        • MGC119185 antibody
        • MGC189143 antibody
        • mogat2 antibody
        • MOGT2_HUMAN antibody
        • Monoacylglycerol O acyltransferase 1 like antibody
        • Monoacylglycerol O acyltransferase 2 antibody
        • Monoacylglycerol O-acyltransferase 2 antibody
        see all

      Images

      • Western blot - Anti-MGAT2 antibody (ab228950)
        Western blot - Anti-MGAT2 antibody (ab228950)
        Anti-MGAT2 antibody (ab228950) at 1/3000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

        Developed using the ECL technique.

        Predicted band size: 38 kDa



        10% SDS-PAGE

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab228950? Please let us know so that we can cite the reference in this datasheet.

      ab228950 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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