Overview

  • Product name

    Anti-MGAT3 antibody - N-terminal
    See all MGAT3 primary antibodies
  • Description

    Rabbit polyclonal to MGAT3 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, selected from the N-terminal region of Human MGAT3.

  • Positive control

    • Mouse spleen tissue lysate; Human breast carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab135514 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab135514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 61 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 17 family.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase antibody
    • Beta 1 4 mannosyl glycoprotein 4 beta N acetylglucosaminyltransferase antibody
    • Beta-1 antibody
    • FLJ43371 antibody
    • GlcNAc T III antibody
    • GlcNAc-T III antibody
    • GNT 3 antibody
    • GNT III antibody
    • GNT-III antibody
    • GNT3 antibody
    • Mannosyl (beta 1 4 ) glycoprotein beta 1 4 N acetylglucosaminyltransferase antibody
    • MGAT 3 antibody
    • MGAT3 antibody
    • MGAT3_HUMAN antibody
    • MGC141943 antibody
    • MGC142278 antibody
    • N acetylglucosaminyltransferase III antibody
    • N glycosyl oligosaccharide glycoprotein N acetylglucosaminyltransferase III antibody
    • N-acetylglucosaminyltransferase III antibody
    • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III antibody
    • OTTHUMP00000042207 antibody
    see all

Images

  • Anti-MGAT3 antibody - N-terminal (ab135514) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg

    Predicted band size: 61 kDa

  • Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue labelling MGAT3 with ab135514 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and AEC staining.

References

This product has been referenced in:

  • Tan Z  et al. Bisecting N-Acetylglucosamine Structures Inhibit Hypoxia-Induced Epithelial-Mesenchymal Transition in Breast Cancer Cells. Front Physiol 9:210 (2018). Read more (PubMed: 29593568) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice Hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice Hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Apr 02 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 27 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Aug 18 2015

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