Overview

  • Product name

  • Description

    Mouse monoclonal to MGAT5
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster
  • Immunogen

    Recombinant fragment: LAEPGQSCKQ VCQESQLICE PSFFQHLNKD KDMLKYKVTC QSSELAKDIL VPSFDPKNK HCVFQGDLLL FSCAGAHPRH QRVCPCRDFI KGQVALCKD, corresponding to amino acids 642 - 739 of Human MGAT5 (NP_002401) with a proprietary tag

  • Positive control

    • Jurkat whole cell lysate (ab7899)
  • General notes

    This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab87977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 18 family.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A antibody
    • 6-N-acetylglucosaminyltransferase antibody
    • Alpha 1,3(6) mannosylglycoprotein antibody
    • Alpha 1,6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase antibody
    • Alpha mannoside beta 1,6 N acetylglucosaminyltransferase antibody
    • Alpha-1 antibody
    • Alpha-mannoside beta-1 antibody
    • Beta 1,6 N acetyl glucosaminyltransferase antibody
    • GGNT5 antibody
    • GlcNAc T V antibody
    • GlcNAc-T V antibody
    • GNT V antibody
    • GNT VA antibody
    • GNT-V antibody
    • GNTV antibody
    • GNTVA antibody
    • Mannoside acetylglucosaminyltransferase 5 antibody
    • Mannosyl (alpha 1,6) glycoprotein beta 1,6 N acetyl glucosaminyltransferase antibody
    • Mgat5 antibody
    • MGT5A_HUMAN antibody
    • N acetylglucosaminyl transferase V antibody
    • N acetylglucosaminyltransferase V mannosyl (alpha 1,6) glycoprotein antibody
    • N-acetylglucosaminyl-transferase V antibody
    see all

Images

  • Anti-MGAT5 antibody (ab87977) at 1/2000 dilution + Human RWPE prostate cells at 20 µg

    Secondary
    Donkey anti-mouse polyclonal HRP conjugate at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 85 kDa
    Additional bands at: 55 kDa (possible non-specific binding), 95 kDa (possible post-translational modification)


    Exposure time: 40 seconds


    Blocking was via 5% milk in PBST incubated for 1 hour at 22°C

    This image was generated using the ascites version of the product.

    See Abreview

  • Overlay histogram showing Jurkat cells stained with ab87977 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87977, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the ascites version of the product.

  • Anti-MGAT5 antibody (ab87977) at 1 µg/ml + recombinant protein fragment with a proprietary tag (the immunogen) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size: 85 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    The recombinant protein used as the immunogen has a predicted size of 37 kDa.

    This image was generated using the ascites version of the product.

  • Anti-MGAT5 antibody (ab87977) at 1 µg/ml + jurkat cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 85 kDa



    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Yan G  et al. Decreased miR-124-3p promoted breast cancer proliferation and metastasis by targeting MGAT5. Am J Cancer Res 9:585-596 (2019). Read more (PubMed: 30949412) »
  • Wang G  et al. Loss of Barx1 promotes hepatocellular carcinoma metastasis through up-regulating MGAT5 and MMP9 expression and indicates poor prognosis. Oncotarget 8:71867-71880 (2017). Read more (PubMed: 29069753) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice Hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice Hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Apr 02 2019

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mice Hepatocytes)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
35 µg
Specification
Mice Hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Feb 19 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Nov 27 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (RWPE - prostate cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
20 µg
Specification
RWPE - prostate cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Oct 09 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PC-3 prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
PC-3 prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Aug 18 2015

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