Recombinant
RabMAb

Anti-MGEA5 antibody [EPR7154(B)] (ab124807)

Overview

  • Product name
    Anti-MGEA5 antibody [EPR7154(B)]
    See all MGEA5 primary antibodies
  • Description
    Rabbit monoclonal [EPR7154(B)] to MGEA5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human MGEA5. The exact sequence is proprietary.
    (Peptide available as ab155521)

  • Positive control
    • HeLa, BxPC-3, JAR and fetal brain lysates; Human kidney tissue
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 130 kDa (predicted molecular weight: 102 kDa).Can be blocked with MGEA5 peptide (ab155521).
IP 1/100 - 1/500.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
      Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
    • Tissue specificity
      Ubiquitous. Shows highest expression in the brain, placenta and pancreas.
    • Sequence similarities
      Belongs to the glycosyl hydrolase 84 family.
    • Post-translational
      modifications
      Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.
    • Cellular localization
      Cytoplasm and Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta-hexosaminidase antibody
      • Beta-N-acetylglucosaminidase antibody
      • Beta-N-acetylhexosaminidase antibody
      • Bifunctional protein NCOAT antibody
      • HEXC antibody
      • Hyaluronidase in meningioma antibody
      • KIAA0679 antibody
      • MEA5 antibody
      • Meningioma expressed antigen 5 antibody
      • Meningioma-expressed antigen 5 antibody
      • MGEA 5 antibody
      • Mgea5 antibody
      • N-acetyl-beta-D-glucosaminidase antibody
      • N-acetyl-beta-glucosaminidase antibody
      • NCOAT antibody
      • Nuclear cytoplasmic O GlcNAcase and acetyltransferase antibody
      • Nuclear cytoplasmic O-GlcNAcase and acetyltransferase antibody
      • OGA antibody
      • OGA_HUMAN antibody
      • Protein O-GlcNAcase antibody
      see all

    Images

    • ab124807, at 1/100 dilution, staining MGEA5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • All lanes : Anti-MGEA5 antibody [EPR7154(B)] (ab124807) at 1/10000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : BxPC-3 lysate
      Lane 3 : JAR lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 102 kDa
      Observed band size: 130 kDa (why is the actual band size different from the predicted?)

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Shi H  et al. Skeletal muscle O-GlcNAc transferase is important for muscle energy homeostasis and whole-body insulin sensitivity. Mol Metab N/A:N/A (2018). Read more (PubMed: 29525407) »
    • Zhang B  et al. Bitterness in sugar: O-GlcNAcylation aggravates pre-B acute lymphocytic leukemia through glycolysis via the PI3K/Akt/c-Myc pathway. Am J Cancer Res 7:1337-1349 (2017). WB ; Human . Read more (PubMed: 28670495) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    As discussed in my previous email, I have now arranged for the immunizing peptide to be available from our catalogue. This can be viewed under the catalogue number ab155521:

    https://www.abcam.com/ab155521

    I hope this information has b...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic stem cell)
    Loading amount
    20 µg
    Specification
    Mouse embryonic stem cell
    Gel Running Conditions
    Reduced Denaturing (4-12%, MOPS buffer)
    Blocking step
    Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted May 07 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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