Key features and details
- Rabbit polyclonal to MGEA5/OGA
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MGEA5/OGA antibody
See all MGEA5/OGA primary antibodies
DescriptionRabbit polyclonal to MGEA5/OGA
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Cow, Dog, Pig, Macaque monkey, Gorilla
Synthetic peptide within Human MGEA5/OGA aa 500-600 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: U87mg; JEG3; PDX1.
Previously labelled as MGEA5
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab105217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionIsoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
Tissue specificityUbiquitous. Shows highest expression in the brain, placenta and pancreas.
Sequence similaritiesBelongs to the glycosyl hydrolase 84 family.
modificationsProteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.
Cellular localizationCytoplasm and Nucleus.
- Information by UniProt
- Beta-hexosaminidase antibody
- Beta-N-acetylglucosaminidase antibody
- Beta-N-acetylhexosaminidase antibody
All lanes : Anti-MGEA5/OGA antibody (ab105217) at 1 µg
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MGEA5 knockout HAP1 whole cell lysate
Lane 3 : U20S whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 103 kDa
Lanes 1 - 4: Merged signal (red and green). Green - ab105217 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab105217 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in MGEA5 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and MGEA5 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab105217 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab105217 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab105217 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-MGEA5/OGA antibody (ab105217) at 1 µg/ml
Lane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 3 : PDX1(Pancreatic and duodenal homeobox 1) Whole Cell Lysate
Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lane 5 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lane 6 : PDX1(Pancreatic and duodenal homeobox 1) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
ab105217 has been referenced in 1 publication.
- Huang L et al. O-GlcNAc-modified SNAP29 inhibits autophagy-mediated degradation via the disturbed SNAP29-STX17-VAMP8 complex and exacerbates myocardial injury in type I diabetic rats. Int J Mol Med 42:3278-3290 (2018). PubMed: 30221662