Anti-MGEA5/OGA antibody (ab197389)
Key features and details
- Rabbit polyclonal to MGEA5/OGA
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MGEA5/OGA antibody
See all MGEA5/OGA primary antibodies -
Description
Rabbit polyclonal to MGEA5/OGA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human MGEA5/OGA (internal sequence).
Database link: O60502 -
Positive control
- Human fetal liver tissue lysate. Human colon cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/1000. Predicted molecular weight: 103 kDa.
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IHC-P |
1/25 - 1/100.
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Notes |
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WB
1/200 - 1/1000. Predicted molecular weight: 103 kDa. |
IHC-P
1/25 - 1/100. |
Target
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Function
Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1. -
Tissue specificity
Ubiquitous. Shows highest expression in the brain, placenta and pancreas. -
Sequence similarities
Belongs to the glycosyl hydrolase 84 family. -
Post-translational
modificationsProteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity. -
Cellular localization
Cytoplasm and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10724 Human
- Entrez Gene: 76055 Mouse
- Entrez Gene: 154968 Rat
- Omim: 604039 Human
- SwissProt: O60502 Human
- SwissProt: Q9EQQ9 Mouse
- SwissProt: Q8VIJ5 Rat
- Unigene: 500842 Human
see all -
Alternative names
- Beta-hexosaminidase antibody
- Beta-N-acetylglucosaminidase antibody
- Beta-N-acetylhexosaminidase antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab197389 has not yet been referenced specifically in any publications.