Key features and details
- Rabbit polyclonal to MGEA5/OGA
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MGEA5/OGA antibody
See all MGEA5/OGA primary antibodies
DescriptionRabbit polyclonal to MGEA5/OGA
This antibody detects endogenous levels of total MGEA5/OGA protein.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human MGEA5/OGA (internal sequence).
Database link: O60502
- Human fetal liver tissue lysate. Human colon cancer tissue.
Previously labelled as MGEA5
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/1000. Predicted molecular weight: 103 kDa.|
|IHC-P||1/25 - 1/100.|
FunctionIsoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
Tissue specificityUbiquitous. Shows highest expression in the brain, placenta and pancreas.
Sequence similaritiesBelongs to the glycosyl hydrolase 84 family.
modificationsProteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.
Cellular localizationCytoplasm and Nucleus.
- Information by UniProt
- Beta-hexosaminidase antibody
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ab197389 has not yet been referenced specifically in any publications.